Exploring the Mechanism of Chang-An-Kang on Active Ulcerative Colitis Rats Based on NF-κB/STAT3 Signaling Pathway
Zhenhua Lu1★, Gangjie Wei1, Qing Xu1, Yangguang Li1, Xiaojun Cai1★ Corresponding author
- 1Department of Hepatobiliary, Spleen and Gastroenterology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin, China.
CORRESPONDENCE
Zhenhua Lu
Department of Hepatobiliary, Spleen and Gastroenterology. Heilongjiang Academy of Traditional Chinese Medicine, Harbin, Heilongjiang 150036, China.
Received: 14-11-2023; Accepted: 18-12-2023.
Volume 14, Issue 4 · pp. 268–274 · PUBLISHED 2023 · DOI: 10.1177/0976500X231224907
View on J Pharmacol. Pharmacother. original site ↗
ABSTRACT
Objectives: The objective of this study was to investigate the therapeutic effect and mechanism of Chang-An-Kang on ulcerative colitis rats. Materials and Methods: The rat model of ulcerative colitis induced by 6% glacial acetic acid was established. The corresponding therapeutic drugs were given 24 h after the model was established. The pathological morphology of colon tissue was observed after 21 days of continuous administration. The levels of interleukin-6 (IL-6), interleukin-7 (IL-7), interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNF-α) were measured by ELISA kit. The expression levels of NF-κB and STAT3 proteins in colon tissue were detected by IHC. Results: The results showed that the changes in the pathological and biochemical indexes of the rat colon tissue indicate that the model was successfully established. Chang-An-Kang could significantly reduce rat colon tissue damage, decrease the content of IL-6, IL-7, and TNF-α, and increase the content of IL-10. The NF-κB and STAT3 protein expression levels were decreased. Conclusion: By increasing the content of anti-inflammatory factor IL-10, decreasing the content of pro-inflammatory factors IL-6 and TNF-α, and inhibiting the expression of NF-κB and STAT3 proteins, Chang-An-Kang can reduce inflammatory infiltration. It has an obvious therapeutic effect on acetic acid ulcerative colitis in rats.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Lu, Z., Wei, G., Xu, Q., Li, Y., & Cai, X. (2023). Exploring the Mechanism of Chang-An-Kang on Active Ulcerative Colitis Rats Based on NF-κB/STAT3 Signaling Pathway. Journal of Pharmacology and Pharmacotherapeutics, 14(4), 268–274. https://doi.org/10.1177/0976500X231224907