Evaluation of Genetic Diversity in Cardiospermum halicacabum based on Geographical Variation Using RAPD Markers
Annamalai A1★, Christina V.L1, Lakshmi P.T.V.2★ Corresponding author
- 1Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore, Tamil Nadu, INDIA.
- 2Center for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, INDIA.
CORRESPONDENCE
Annamalai A
Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore, Tamil Nadu, INDIA.
Volume 2, Issue 1 · pp. 62–65 · PUBLISHED · DOI: 10.5530/pc.2012.1.11
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ABSTRACT
Background:Genetic diversity at the intra species level arises from differences in climatic conditions, geographical distribution and environmental stress. Thus the study of genetic diversity assists in determining the phylogeny of a particular plant species and the impact of geographical locations on the plant genome. Objective: To assess the genetic diversity and phylogeny of Cardiospermum halicacabumspecies’ from different regions within the state of Tamil Nadu in India. Materials and Methods: Plant DNA was isolated and amplified using eight random primers. The presence of amplified DNA bands was recorded as a 1, while the absence of any bands was recorded as a 0. The percentages of polymorphism were determined using the ratio of similarity to dissimilarity of band pattern. The data was analysed using the VisionWork LS Analysis Software to construct a dendrogram to create a phylogenetic tree, for cluster analysis using UPGMA (Unweigthed Pair Group Method with Arithmetical Mean). Results: RAPD analysis showed varied amplifications for each sample. The polymorphism was 29.41% and the number of polymorphic fragments ranged from 603 bp to 6557 bp. The dendrogram revealed two clusters, Cluster I included the sample from Chennai, while Cluster II included samples from Dharmapuri, Thanjavur, Thirunalveli, Coimbatore and Madurai, thus showing the extent of intraspecific variation. Conclusion: Variation in geographical plant location was consistent with genetic differences. Therefore DNA banding patterns may be used as a fingerprint marker for the identification of Cardiospermum halicacabum species.
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A, A., V.L, C., & P.T.V., L. (). Evaluation of Genetic Diversity in Cardiospermum halicacabum based on Geographical Variation Using RAPD Markers. Pharmacognosy Communications, 2(1), 62–65. https://doi.org/10.5530/pc.2012.1.11