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    Pharmacognosy CommunicationsNOW IN
    Article

    Optimisation Of Solvent Extraction Of Antioxidants (Phenolic Compounds) From Algerian Mint ( Mentha spicata L. )

    Brahmi Fatiha1★, Madani Khodir1, Dahmoune Farid1, Rahmani Tiziri1, Bousbaa Karima1, Sonia Oukmanou1, Chibane Mohamed1★ Corresponding author

    1. 1bs Laboratory, Faculty of Life and Nature Sciences, University of Bejaia, Bejaia 06000, ALGERIA.

    CORRESPONDENCE

    Brahmi Fatiha

    3bs Laboratory, Faculty of Life and Nature Sciences, University of Bejaia, Bejaia 06000, ALGERIA.

    fatiha12001@yahoo.fr

    Volume 2, Issue 4 · pp. 72–86 · PUBLISHED · DOI: 10.5530/pc.2012.4.10

    View on Pharmacogn. Commn. original site ↗

    ABSTRACT

    Introduction:The mints are herbs rich in phenolic compounds that appear to be capable of preventing many diseases. This study allowed for quantifation of the levels of total phenolics and flavonoids from Mentha spicata L. harvested in Bejaia (Algeria) using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50 and 75%. Furthermore, the antioxidant levels were evaluated by two methods. Materials and methods: The total phenolics content (TPC) was evaluated by the Folin-Ciocalteu method. Total Flavonoids content (TFC) was determined by aluminium chloride method Radical scavenging activity (RSA) and total antioxidant activity (TAA) of the extracts were determined by standard methods. Results and conclusion: The solvents ethanol and acetone (at 75%) gave the best extraction rates for Mentha spicata (20.02 and 20.20% respectively). The ethanol extract (at 50%) presented the highest TPC (39.47 ± 1.81 mg EAG/g DW). Regarding TFC, acetone 75% was the best extractor (7.68±0 .02 mg EQ/g DW). The acetonic extract at 50% manifests the highest RSA, whereas, the TAA was attributed to the acetonic (0.23 ± 0.01 AU) and ethanolic extracts (0.22 ± 0.02 AU) without signifi cant difference. The variation in the antioxidant capacity between extracts was probably due to the difference of the nature of the compounds extracted with different solvents.

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      Fatiha, B., Khodir, M., Farid, D., Tiziri, R., Karima, B., Oukmanou, S., & Mohamed, C. (). Optimisation Of Solvent Extraction Of Antioxidants (Phenolic Compounds) From Algerian Mint ( Mentha spicata L. ). Pharmacognosy Communications, 2(4), 72–86. https://doi.org/10.5530/pc.2012.4.10