Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy CommunicationsNOW IN
    Article

    Growth Inhibitory Activity of Selected High Antioxidant Australian Syzygium Species Against the Food Poisoning and Tissue Necrotic Pathogen Clostridium perfringens

    Mitchell Henry Wright1, Cameron Jay Lee1, Charmaine Estelle Pollock1, Anthony Carlson Greene1, Ian Edwin Cock1,2★★ Corresponding author

    1. 1School of Natural Sciences, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.
    2. 2Environmental Futures Research Institute, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.

    CORRESPONDENCE

    Ian Edwin Cock

    School of Natural Sciences, Nathan Campus, Griffith University, 170 Kessels Rd, Nathan, Queensland 4111, AUSTRALIA.

    i.cock@griffith.edu.au

    Volume 6, Issue 2 · pp. 93–99 · PUBLISHED · DOI: 10.5530/pc.2016.2.7

    View on Pharmacogn. Commn. original site ↗

    ABSTRACT

    Introduction:Clostridium perfringens is a gram positive pathogen which is an etiological agent in Clostridial myonecrosis and enteritis necroticans. Unless promptly treated, C. perfringens infections may result in tissue necrosis and death. Syzygium australe (brush cherry) and Syzygium lu- ehmannii (riberry) fruit and leaves have documented therapeutic proper- ties as general antiseptic agents against an extensive panel of bacteria. Despite this, studies are yet to test the growth inhibitory activity of these species against C. perfringens. Methods:S. australe and S. luehmannii fruit and leaf extracts were investigated by disc diffusion assay for growth inhibitory activity against a clinical strain of Clostridium perfringens. Their MIC values were determined to quantify and compare their efficacies. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results:Methanolic and aqueous S. australe and S. luehmannii fruit and leaf extracts as well as the corresponding fruit ethyl acetate extracts, displayed growth inhibitory activity in the disc diffusion assay against C. perfringens. The aqueous and methanolic extracts were particularly potent growth inhibitors, each with MIC values substantially <500 µg/mL. The S. australe fruit extracts were nontoxic in the Artemia franciscana bioassay (LC50 values <1000 µg/mL). All ethyl acetate extracts were also nontoxic. In contrast, the S. luehmannii aqueous and methanolic extracts (fruit and leaf), as well as the S. australe leaf extracts displayed substantial toxicity in the assay. Conclusion:The potent growth inhibitory bioactivity of the fruit and leaf aqueous and methanolic Syzygium spp. extracts against C. perfringens indicates their potential as medicinal agents in the treatment and prevention of clostridial myonecrosis and enteritis necroticans.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Wright, M. H., Lee, C. J., Pollock, C. E., Greene, A. C., & Cock, I. E. (). Growth Inhibitory Activity of Selected High Antioxidant Australian Syzygium Species Against the Food Poisoning and Tissue Necrotic Pathogen Clostridium perfringens. Pharmacognosy Communications, 6(2), 93–99. https://doi.org/10.5530/pc.2016.2.7