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    High-performance liquid chromatography analysis of plant saponins: An update 2005-2010

    Jagmohan S. Negi1,2★, Pramod Singh1, Geeta Joshi Nee Pant1, M. S. M. Rawat1★ Corresponding author

    1. 1Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal).
    2. 2Herbal Research and Development Institute, Gopeshwar (Chamoli), Uttarakhand, INDIA.

    CORRESPONDENCE

    Jagmohan S. Negi

    Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal).

    negi_js1981@yahoo.co.in

    Received: 17-07-2010; Revised: 04-04-2011; Accepted: 10-10-2011.

    Volume 5, Issue 10 · pp. 155–158 · PUBLISHED 23 December 2011 · DOI: 10.4103/0973-7847.91109

    View on Pharmacogn. Rev. original site ↗

    ABSTRACT

    Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector, evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.

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      Negi, J. S., Singh, P., Pant, G. J. N., & Rawat, M. S. M. (2011). High-performance liquid chromatography analysis of plant saponins: An update 2005-2010. Pharmacognosy Reviews, 5(10), 155–158. https://doi.org/10.4103/0973-7847.91109