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    Increased acetylcholine esterase activity produced by the administration of an aqueous extract of the seed kernel of Thevetia peruviana and its role on acute and subchronic intoxication in mice

    Rubén Marroquín‑Segura2★, Ricardo Calvillo‑Esparza2, José Luis Alfredo Mora‑Guevara2, José Horacio Tovalín‑Ahumada2, Abigail Aguilar‑Contreras3, Vicente Jesús Hernández‑Abad2,4★ Corresponding author

    1. 1Laboratorio de Inmunología, Unidad Multidisciplinaria de Investigación Experimental, División de Estudios de Posgrado e Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México. Batalla de 5 de mayo s/n, Col. Ejército de Oriente, CP 09230, Mexico.
    2. 2Herbario de plantas medicinales, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social. Av. Cuauhtemoc 330, Col. Doctores, CP 06725, Mexico.
    3. 3Laboratorio de Investigación Farmacéutica, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México. Batalla de 5 de mayo s/n, Col. Ejército de Oriente, CP 09230, Mexico City, Mexico.

    CORRESPONDENCE

    Rubén Marroquín‑Segura

    Herbario de plantas medicinales, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social. Av. Cuauhtemoc 330, Col. Doctores, CP 06725, Mexico.

    Received: 30-09-2012; Revised: 11-11-2012.

    phm-v10-i37 · pp. S171–S175 · PUBLISHED 21 February 2014 · DOI: 10.4103/0973-1296.127370

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The real mechanism for Thevetia peruviana poisoning remains unclear. Cholinergic activity is important for cardiac function regulation, however, the effect of T. peruviana on cholinergic activity is not well‑known. Objective: To study the effect of the acute administration of an aqueous extract of the seed kernel of T. peruviana on the acetylcholine esterase (AChE) activity in CD1 mice as well its implications in the sub‑chronic toxicity of the extract. Materials and Methods: A dose of 100 mg/kg of the extract was administered to CD1 mice and after 7 days, serum was obtained for ceruloplasmin (CP) quantitation and liver function tests. Another group of mice received a 50 mg/kg dose of the extract 3 times within 1 h time interval and AChE activity was determined for those animals. Heart tissue histological preparation was obtained from a group of mice that received a daily 50 mg/kg dose of the extract by a 30‑days period. Results: CP levels for the treated group were higher than those for the control group (Student’s t‑test, P ≤ 0.001). AChE activity in the treated group was significantly higher than the control group (Tukey test, control vs. T. peruviana, P ≤ 0.001). Heart tissue histological preparations showed leukocyte infiltrates and necrotic areas, consistent with infarcts. Conclusion: The increased levels of AChE and the hearth tissue infiltrative lesions induced by the aqueous seed kernel extract of T. peruviana explains in part the poisoning caused by this plant, which can be related to an inflammatory process.

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      Marroquín‑Segura, R., Calvillo‑Esparza, R., Mora‑Guevara, J. L. A., Tovalín‑Ahumada, J. H., Aguilar‑Contreras, A., & Hernández‑Abad, V. J. (2014). Increased acetylcholine esterase activity produced by the administration of an aqueous extract of the seed kernel of Thevetia peruviana and its role on acute and subchronic intoxication in mice. Pharmacognosy Magazine, , S171–S175. https://doi.org/10.4103/0973-1296.127370