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    Dichlorodiaportinol A – A new chlorine‑containing isocoumarin from an endophytic fungus Trichoderma sp. 09 from Myoporum bontioides A. Gray and its cytotoxic activity

    Chunyuan Li1★, Bing Gong1, Daniel G. Cox2, Caili Li3, Jinhua Wang1, Weijia Ding1★ Corresponding author

    1. 1Institute of Biomaterial, College of Science, South China Agricultural University, Guangzhou, China.
    2. 2Department of Pharmacognosy and Research Institute of Pharmaceutical Sciences, School of Pharmacy, the University of Mississippi, Mississippi University, Mississippi, USA.
    3. 3Xinyang Branch, Henan Provincial academy of Agricultural Sciences, Xinyang, China.

    CORRESPONDENCE

    Chunyuan Li

    Institute of Biomaterial, College of Science, South China Agricultural University, Guangzhou, China.

    Received: 10-06-2013; Revised: 29-06-2013.

    Volume 10, Issue 37s · pp. · PUBLISHED 21 February 2014 · DOI: 10.4103/0973-1296.127367

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Myoporum bontioides A. Gray, an evergreen shrub from the Myoporaceae family, is a commonly used medicinal plant. Many studies have been conducted on the biologically active constituents of whole parts of M. bontioides. However, the endophytes of M. bontioides have not been intensively investigated. A new chlorine‑containing isocoumarin, named dichlorodiaportinol A (1) was isolated from the endophytic fungus Trichoderma sp. 09 isolated from the root of M. bontioides. Its cytotoxic activity against human breast cancer (MCF‑7) and human liver cancer (HepG2) cell lines was evaluated. Materials and Methods: Different open silica gel column chromatographic techniques with different solvent systems were used for the separation of the constituents of the ethyl acetate extract of the culture broth of the endophytic fungus Trichoderma sp. 09. The structure of compound one was identified by analysis of spectroscopic data [one‑dimensional (1D), two‑dimensional (2D)‑nuclear magnetic resonance (NMR), ultraviolet (UV), infrared (IR) and Mass spectrometry (MS)]. 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑Diphenyltetrazolium Bromide (MTT) assay method was used for the evaluation of cytotoxic activity of compound one against MCF‑7 and HepG2 cell lines. Results: Compound one was identified as 3‑(3,3‑dichloro‑2,3‑dihydroxy‑propyl)‑8‑hydroxy‑6‑ methoxy‑isochromen‑1‑one. It inhibited MCF‑7 and HepG2 cell lines, with half maximal inhibitory concentration (IC50) values of 17.8 and 39.6 μg/mL, respectively. Conclusions: Compound one is a new chlorine‑containing isocoumarin with moderate cytotoxic activity against MCF‑7 and HepG2 cell lines. Thus, endophytes of M. bontioides are worthy of consideration for the development and research of antitumor agents.

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      Li, C., Gong, B., Cox, D. G., Li, C., Wang, J., & Ding, W. (2014). Dichlorodiaportinol A – A new chlorine‑containing isocoumarin from an endophytic fungus Trichoderma sp. 09 from Myoporum bontioides A. Gray and its cytotoxic activity. Pharmacognosy Magazine, 10(37s). https://doi.org/10.4103/0973-1296.127367