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    Flavonols and derivatives of gallic acid from young leaves of Toona sinensis (A. Juss.) Roemer and evaluation of their anti-oxidant capacity by chemical methods

    Huan Yang2, Qinying Gu2, Tingting Gao2, Xubo Wang3, Phenwei Chue2, Qinan Wu4, Xiaobin Jia2,5★★ Corresponding author

    1. 1Department of Chinese Materia Medica, School of Pharmacy, Jiangsu University.
    2. 2Department of Pharmaceutical Technology and Biological Engineering, Changzhou Institute of Engineering Technology.
    3. 3Department of Pharmacognosy, School of Pharmacy, Nanjing University of Chinese Medicine.
    4. 4Department of Pharmacy, Key Lab of Chinese Medicine Delivery System of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, School of Pharmacy, Jiangsu University, China.

    CORRESPONDENCE

    Xiaobin Jia

    Department of Pharmaceutical Technology and Biological Engineering, Changzhou Institute of Engineering Technology.

    jxiaobin2005@hotmail.com

    Received: 15-02-2013; Revised: 23-02-2013.

    Volume 10, Issue 38 · pp. 185–190 · PUBLISHED 17 April 2014 · DOI: 10.4103/0973-1296.131034

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Toona sinensis (A. Juss.) Roemer is an endemic species of Toona genus native to Asia. Its crude extract exhibits an effective anti-oxidant capacity against oxidative models, but the intrinsic substances responsible for this capacity in the extract remains unclear and is yet to be studied comprehensively. Objective: To investigate the chemical constituents of the young leaves of Toona sinensis and its anti-oxidant capacity. Materials and Methods: Silica gel column chromatography, preparative high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and mass spectrometry (MS) were used to isolate and characterize the chemical constituents. Four chemical-induced oxidative models including DPPH free-radical scavenging assay, phenazine methosulphate (PMS) nicotinamide adenine dinucleotide (NADH) PMS-NADH-NBT superoxide anion scavenging assay, FeCl3-K3Fe (CN) 6 reducing power assay, and FeCl2-FerroZine metal chelation assay were applied in the present study for evaluating anti-oxidant capacity. Results: Five fl avonols and three derivatives of gallic acid, including quercetrin, kaem pferol-3-O-α-L-rhamopyranoside, astragalin, quercetin, kaempferol, methyl gallate, ethyl gallate, and 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucopyranose were isolated from the leaves. Results showed that these compounds exhibited various antioxidant properties, markedly either as the strong scavengers for superoxide and free radicals or as molecules that were reducing or metal chelating in nature. Conclusion: The fi ndings suggested that the 8 compounds in the young leaves of T. sinensis that were isolated in our study were the active compounds responsible for its antioxidant activity. These compounds can be utilized as a potential health supplement, as an available source of natural antioxidants, and as an effective material in pharmaceutical applications.

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      Yang, H., Gu, Q., Gao, T., Wang, X., Chue, P., Wu, Q., & Jia, X. (2014). Flavonols and derivatives of gallic acid from young leaves of Toona sinensis (A. Juss.) Roemer and evaluation of their anti-oxidant capacity by chemical methods. Pharmacognosy Magazine, 10(38), 185–190. https://doi.org/10.4103/0973-1296.131034