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    Article

    Simultaneous quantification and splenocyte‑proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum

    Ying Zong2,3, Yu Wang4, Hang Li2, Na Li2, Hui Zhang2★, Jiaming Sun2, Xiaohui Niu5, Xiaochen Gao2★ Corresponding author

    1. 1Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun, China.
    2. 2College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.
    3. 3Tianjin Tasly Institute, Chinese Botanical R and D Institute, Tianjin, China.
    4. 4School of Chemistry and Life Science, Changchun University of Technology, Changchun, China.

    CORRESPONDENCE

    Hui Zhang

    College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.

    sun_jiaming2000@163.com

    Received: 12-01-2014; Revised: 25-04-2014.

    Volume 10, Issue 40 · pp. 391–397 · PUBLISHED 26 September 2014 · DOI: 10.4103/0973-1296.141757

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Cervi Cornu Pantotrichum has been a well known traditional Chinese medicine, which is young horn of Cervus Nippon Temminck (Hualurong: HLR). At present, the methods used for the quality control of Cervi Cornu Pantotrichum show low specificity. Objective: To describe a holistic method based on chemical characteristics and splenocyte‑proliferating activities to evaluate the quality of HLR. Materials and Methods: The nucleosides and bases from HLR were identified by high performance liquid chromatography electrospray ionization mass spectrometry (HPLC‑ESI‑MS), and six of them were chosen to be used for simultaneous HPLC quantification according to the results of proliferation of mouse splenocytes in vitro. Results: In this study, eight nucleosides and bases have been identified. In addition, uracil, hypoxanthine, uridine, inosine, guanosine, and adenosine were chosen to be used for simultaneous HPLC quantification. Simultaneous quantification of these six substances was performed on ten groups of HLR under the condition of a TIANHE Kromasil C18 column (5 μm, 4.6 mm × 250 mm i.d.) and a gradient elution of water and acetonitrile. Of the ten groups, HLR displayed the highest total nucleoside contents (TNC, sum of adenosine and uracil, 0.412 mg/g) with the strongest splenocyte‑proliferating activities. Conclusion: These results suggest that TNC (such as particularly highly contained adenosine and uracil) in HLR has a certain correlation with the activity of splenocyte‑proliferating, and it may be used as a quality control for HLR. This comprehensive method could be applied to other traditional Chinese medicines to ameliorate their quality control.

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      Zong, Y., Wang, Y., Li, H., Li, N., Zhang, H., Sun, J., Niu, X., & Gao, X. (2014). Simultaneous quantification and splenocyte‑proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum. Pharmacognosy Magazine, 10(40), 391–397. https://doi.org/10.4103/0973-1296.141757