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    Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC‑DAD

    P.R. Rao Vadaparthi1, K. Kumar1, Vanka Uma Maheswara Sarma1, Qazi Altaf Hussain2, K. Suresh Babu1★★ Corresponding author

    1. 1Division of Natural Products Chemistry, CSIR‑Indian Institute of Chemical Technology, Hyderabad, India.
    2. 2Department of Horticulture, Faculty of Agriculture, Sher‑e‑Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore, J and K, India.

    CORRESPONDENCE

    K. Suresh Babu

    Division of Natural Products Chemistry, CSIR‑Indian Institute of Chemical Technology, Hyderabad, India.

    suresh@iict.res.in

    Received: 14-05-2014; Revised: 12-06-2014.

    Volume 11, Issue 41 · pp. 180–190 · PUBLISHED 21 January 2015 · DOI: 10.4103/0973-1296.149736

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Saussurea lappa is one of the popular Ayurvedic herb; costunolide and dehydrocostus lactones are well‑known sesquiterpene lactones contained in many plants used as popular herbs, such as S. lappa, and have been considered as potential candidates for the treatment of various types of tumor. Objective: The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants. Materials and Methods: HPLC analysis was performed on Waters NOVAPAK HR C18 column (300 mm × 3.9 mm i.d., 6 µm) using isocratic elution with acetonitrile and water (60:40% v/v). Results: The calibration curves of both analytes showed good linearity within the established range 5‑100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3‑101.8%). Conclusion: The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products.

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      Vadaparthi, P. R., Kumar, K., Sarma, V. U. M., Hussain, Q. A., & Babu, K. S. (2015). Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC‑DAD. Pharmacognosy Magazine, 11(41), 180–190. https://doi.org/10.4103/0973-1296.149736