Promoting effect of triterpenoid compound from Agrimonia pilosa Ledeb on preadipocytes differentiation via up‑regulation of PPARγ expression
Tingwang Guo1, Liancai Zhu1★, Jun Tan2, Xuemei Zhou1, Ling Xiao1, Xi Liu1, Bochu Wang1★ Corresponding author
- 1Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, China.
- 2School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, China.
CORRESPONDENCE
Liancai Zhu
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, China.
Received: 22-12-2013.
Volume 11, Issue 41 · pp. 219–225 · PUBLISHED 21 January 2015 · DOI: 10.4103/0973-1296.149741
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ABSTRACT
Background: Agrimonia Pilosa Ledeb (APL), a traditional Chinese medicine, has been reported a variety of biological activities, including treating T2DM. Objective: Triterpenoid compound (TC) was collected from APL. The aim of this study was to investigate the effects of TC on 3T3‑L1 preadipocytes differentiation and genes related to differentiation and IR. Materials and Methods: Column chromatography was used to collect TC from ALP. 3T3‑L1 cell differentiation was induced typically in the presence of various concentrations of TC or pioglitazone. Oil red O staining and measurement of intracellular TG content were performed on the seventh day of differentiation. Then quantitative polymerase chain reaction (Q‑PCR) was used to test the expressions of three transcription factors （PPARγ, CCAAT enhancer binding protein‑α (C/EBP‑α), and sterol regulatory element‑binding protein 1 (SREBP‑1)） and the target genes of PPARγ including glucose transporter (GLUT4), lipoprotein lipase (LPL), fat acid binding protein (AP2), and adiponectin in 3T3‑L1 cells. Results: At the concentration of 5, 25 and 125 μg/mL, TC significantly promoted triglyceride accumulation. Further study showed that TC could promote the expression of PPARγ, C/EBPα and ADD1/SREBP1 significantly at 125 μg/mL. As for downstream genes controlled by PPARγ, TC at 25 and 125 μg/mL could significantly promote the expression of GLUT4 and adiponectin. However, the expression of aP2 related to lipid metabolism and adiposity in the TC group was significantly lower than that in the pioglitazone group. Conclusion: TC could promote preadipocytes differentiation through activating PPARγ and downstream controlled genes. TC has the ideal insulin sensitization with lower adipogenic action than classical TZDs in vitro. So TC from Agrimonia Pilosa Ledeb has a good prospect as a natural drug for IR and T2DM.
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Guo, T., Zhu, L., Tan, J., Zhou, X., Xiao, L., Liu, X., & Wang, B. (2015). Promoting effect of triterpenoid compound from Agrimonia pilosa Ledeb on preadipocytes differentiation via up‑regulation of PPARγ expression. Pharmacognosy Magazine, 11(41), 219–225. https://doi.org/10.4103/0973-1296.149741