Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method

    Gireesh Ankad1, Vinayak Upadhya1, Sandeep R. Pai2★, Mansingraj S. Nimbalkar3, Harsha V. Hegde1,2, Rajesh K. Joshi4, Sanjiva D. Kholkute1,2,4★ Corresponding author

    1. 1Ethnomedicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India.
    2. 2Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India.
    3. 3Department of Botany, Shivaji University, Kolhapur, Maharashtra State, India.
    4. 4Phytochemistry Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India.

    CORRESPONDENCE

    Sandeep R. Pai

    Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belgaum, Karnataka State, India.

    drpaisr@gmail.com

    Received: 17-02-2014; Revised: 27-03-2014; Accepted: 21-01-2015.

    Volume 11, Issue 41 · pp. 90–91 · PUBLISHED 21 January 2015 · DOI: 10.4103/0973-1296.149712

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Nothapodytes nimmoniana (Grah.) Mabb. is a high valued medicinal plant endemic to Western Ghats of India, distributed in fragmented populations. The plant is valued for potent anticancer drug camptothecin (CPT). Objective: The study compares and expounds variation in CPT content from leaves and stems of N. nimmoniana obtained from three populations of Western Ghats, India. The study also describes a method for categorizing these populations using content range chart (CRC) method for percent yield of CPT. Materials and Methods: A total of 60 samples were investigated including ten each of leaves and stems from three localities. Micro-extraction method was implemented to extract CPT. reversed phase ultra-performance liquid chromatography photo diode array technique was used to quantify CPT. Results: Leaf samples of an individual collected from Joida, yielded lowest CPT content (0.002 ± 0.000 g/100 g), whereas a stem sample from Amgaon, yielded highest CPT content (0.123 ± 0.006 g/100 g). The findings suggest great variation in individuals producing and accumulating CPT. Using this data along with earlier published work, five categories of CPT yielding plants were made viz. I: Very low: <0.020, II: Low: 0.021-0.039, III: Moderate: 0.040-0.059, IV: High: 0.060-0.079 and V: Very high: >0.080. Based on CPT content in leaves, majority of individuals were under very low category (Ist) and on the other hand stem samples were in ‘II’ category. Besides, very few individuals were observed in category ‘V’. Conclusion: The study expounds use of CRC method for identifying elite population and suggests the need for its conservation.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Ankad, G., Upadhya, V., Pai, S. R., Nimbalkar, M. S., Hegde, H. V., Joshi, R. K., & Kholkute, S. D. (2015). Evaluating Nothapodytes nimmoniana population from three localities of Western Ghats using camptothecin as phytochemical marker and selection of elites using a new-content range chart method. Pharmacognosy Magazine, 11(41), 90–91. https://doi.org/10.4103/0973-1296.149712