Simultaneous determination three phytosterol compounds, campesterol, stigmasterol and daucosterol in Artemisia apiacea by high performance liquid chromatography‑diode array ultraviolet/visible detector
Jiwoo Lee2, Jin Bae Weon2, Bo‑Ra Yun2, Min Rye Eom2, Choong Je Ma2,3★★ Corresponding author
- 1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200‑701, Korea.
- 2Department of Biomaterials Engineering, Institute of Biotechnology, Kangwon National University, Chuncheon 200‑701, Korea.
CORRESPONDENCE
Choong Je Ma
Department of Biomaterials Engineering, Institute of Biotechnology, Kangwon National University, Chuncheon 200‑701, Korea.
Received: 23-04-2014; Revised: 29-04-2014.
Volume 11, Issue 42 · pp. 297–303 · PUBLISHED 12 March 2015 · DOI: 10.4103/0973-1296.153082
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ABSTRACT
Background: Artemisia apiacea is a traditional herbal medicine using treatment of eczema and jaundice in Eastern Asia, including China, Korea, and Japan. Objective: An accurate and sensitive analysis method using high performance liquid chromatography‑diode array ultraviolet/visible detector and liquid chromatography–mass spectrometry for the simultaneous determination of three phytosterol compounds, campesterol, stigmasterol and daucosterol in A. apiacea was established. Materials and Methods: The analytes were separated on a Shiseido C18 column (5 µm, 4.6 mm I.D. ×250 mm) with gradient elution of 0.1% trifluoroacetic acid and acetonitrile. The flow rate was 1 mL/min and detection wavelengths were set at 205 and 254 nm. Results: Validation of the method was performed to demonstrate its linearity, precision and accuracy. The calibration curves showed good linearity (R2 > 0.9994). The limits of detection and limits of quantification were within the ranges 0.55–7.07 µg/mL and 1.67–21.44 µg/mL, respectively. And, the relative standard deviations of intra‑ and inter‑day precision were <2.93%. The recoveries were found to be in the range of 90.03–104.91%. Conclusion: The developed method has been successfully applied to the analysis for quality control of campesterol, stigmasterol and daucosterol in A. apiacea.
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Lee, J., Weon, J. B., Yun, B., Eom, M. R., & Ma, C. J. (2015). Simultaneous determination three phytosterol compounds, campesterol, stigmasterol and daucosterol in Artemisia apiacea by high performance liquid chromatography‑diode array ultraviolet/visible detector. Pharmacognosy Magazine, 11(42), 297–303. https://doi.org/10.4103/0973-1296.153082