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    Effects of 4‑hydroxyisoleucine from Fenugreek Seeds on Depression‑like Behavior in Socially Isolated Olfactory Bulbectomized Rats

    Padmaja B. Kalshetti1, Ramesh Alluri2, Vishwaraman Mohan3, Prasad Arvind Thakurdesai3★★ Corresponding author

    1. 1Department of Pharmacology, MAEER’S Maharashtra Institute of Pharmacy, Pune, Maharashtra.
    2. 2Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Medak, Andhra Pradesh.
    3. 3Department of Scientific Affairs, Indus Biotech Private Limited, Pune, Maharashtra, Institute of Pharmacy, Pune, Maharashtra, India.

    CORRESPONDENCE

    Prasad Arvind Thakurdesai

    Department of Scientific Affairs, Indus Biotech Private Limited, Pune, Maharashtra, Institute of Pharmacy, Pune, Maharashtra, India.

    prasad@indusbiotech.com

    Volume 11, Issue 44s2 · pp. 388–396 · PUBLISHED 2015 · DOI: 10.4103/0973-1296.168980

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Context: Antidepressant‑like effects of (2S, 3R, 4S)‑4‑hydroxyisoleucine (4‑HI), a major amino acid from fenugreek seeds, has been reported in the animal model of acute depression. Aims: To evaluate effects of subacute administration of 4‑HI in animal model of stress‑induced depression namely socially isolated olfactory bulbectomized rats. Materials and Methods: Bilateral olfactory bulbectomy (OBX) were induced in 30 Sprague‑Dawley rats. After recovery period of 14 days, rats were randomized into five groups of 6 rats each and stressed with social isolation (individual housing). The rats were orally treated with either vehicle (OBX‑Iso), positive control, fluoxetine (30 mg/kg) or 4‑HI (10, 30, 100 mg/kg) once a day from day 14 onward. Separate group of rats with social isolation but without OBX (Sham‑Iso) was also maintained. The behavioral depression and anxiety related parameters using open field test (OFT), sucrose intake test, novelty suppressed feeding (NSF) and forced swim test (FST), and neurochemical estimation (brain monoamines viz., serotonin and nor‑adrenaline, serotonin turnover, and serum cortisol) were performed. Statistical Analysis Used: Data was analyzed by either two‑way ANOVA (OFT and FST) or one‑way ANOVA (sucrose intake test, NSF, and neurochemical estimation) followed by Dunnett’s multiple comparisons test. Differences were considered significant at P < 0.05. Results: The significant and dose‑dependent protection from behavioral and neurochemical changes were observed in 4‑HI co‑administrated OBX‑Iso rats. Conclusion: 4‑HI demonstrated the antidepressant and antianxiety effects in socially isolated stress‑induced OBX rats with possible involvement of multiple stress relieving mechanisms.

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        Kalshetti, P. B., Alluri, R., Mohan, V., & Thakurdesai, P. A. (2015). Effects of 4‑hydroxyisoleucine from Fenugreek Seeds on Depression‑like Behavior in Socially Isolated Olfactory Bulbectomized Rats. Pharmacognosy Magazine, 11(44s2), 388–396. https://doi.org/10.4103/0973-1296.168980