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    Article

    Kinetics of Inhibition of Monoamine Oxidase Using Curcumin and Ellagic Acid

    Dharmendra Kumar Khatri1, Archana Ramesh Juvekar1★★ Corresponding author

    1. 1Department of Pharmaceutical Sciences and Technology, Pharmacology Research Lab-II, Institute of Chemical Technology, (University under Section 3 of UGC Act-1956, Elite Status and Centre of Excellence, Government of Maharashtra, TEQIP Phase II Funded), Mumbai, Maharashtra, India.

    CORRESPONDENCE

    Archana Ramesh Juvekar

    Department of Pharmaceutical Sciences and Technology, Pharmacology Research Lab-II, Institute of Chemical Technology, (University under Section 3 of UGC Act-1956, Elite Status and Centre of Excellence, Government of Maharashtra, TEQIP Phase II Funded), Mumbai, Maharashtra, India.

    juvekar.archana@gmail.com

    Received: 23-09-2015; Revised: 07-12-2015; Accepted: 07-12-2015.

    Volume 12, Issue 46s · pp. S116–20 · PUBLISHED 11 May 2016 · DOI: 10.4103/0973-1296.182168

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Curcumin and ellagic are the natural polyphenols having a wide range of pharmacological actions. They have been reported to have their use in various neurological disorders. Objective: This study was aimed to evaluate the effect of curcumin and ellagic acid on the activity of monoamine oxidase (MAO), the enzyme responsible for metabolism of monoamine neurotransmitters which are pivotal for neuronal development and function. Materials and Methods: The in vitro effects of these selected polyphenols on MAO activities in mitochondria isolated from rat brains were examined. Brain mitochondria were assayed for MAO type-B (MAO-B) using benzylamine as substrates. Rat brain mitochondrial MAO preparation was used to study the kinetics of enzyme inhibition using double reciprocal Lineweaver-Burk plot. Results: MAO activity was inhibited by curcumin and ellagic acid; however, higher half maximal inhibitory concentrations of curcumin (500.46 nM) and ellagic acid (412.24 nM) were required compared to the known MAO-B inhibitor selegiline. It is observed that the curcumin and ellagic acid inhibit the MAO activity with both the competitive and noncompetitive type of inhibitions. Conclusions: Curcumin and ellagic acid can be considered a possible source of MAO inhibitor used in the treatment of Parkinson’s and other neurological disorders.

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      Khatri, D. K., & Juvekar, A. R. (2016). Kinetics of Inhibition of Monoamine Oxidase Using Curcumin and Ellagic Acid. Pharmacognosy Magazine, 12(46s), S116–20. https://doi.org/10.4103/0973-1296.182168