Simultaneous Determination of Eight Bioactive Compounds in Dianthus superbus by High‑performance Liquid Chromatography
Bo-Ra Yun2, Hye Jin Yang2, Jin Bae Weon2, Jiwoo Lee2, Min Rye Eom2, Choong Je Ma2,3★★ Corresponding author
- 1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University.
- 2Department of Biomaterials Engineering, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200‑701, Korea.
CORRESPONDENCE
Choong Je Ma
Department of Biomaterials Engineering, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200‑701, Korea.
Received: 06-07-2015; Revised: 04-08-2015.
Volume 12, Issue 46s · pp. S264–S269 · PUBLISHED 11 May 2016 · DOI: 10.4103/0973-1296.182159
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Dianthus superbus, one of traditional herbal medicine, is widely used to treat urethritis, carbuncles and carcinoma. Objective: A simultaneous determination method was established for controlling the quality of D. superbus using the eight compounds, (E)‑methyl‑4‑hydroxy‑4‑(8a‑methyl‑3‑oxodecahydronaphthalen‑4a‑yl) (1), diosmetin‑7‑O(2’’,6’’‑di‑O‑α‑L‑rhamnopyranosyl)‑β‑D‑glucopyranoside (2), vanillic acid (3), 4‑hydroxyphenyl acetic acid (4), 4‑methoxyphenyl acetic acid (5), (E)‑4‑methoxycinnamic acid (6), 3‑methoxy‑4‑hydroxyphenylethanol (7), and methyl hydroferulate (8) isolated from D. superbus. Materials and Methods: This analysis method was developed using high performance liquid chromatography coupled with diode array detector with a Shishedo C18 column at a column temperature of 3°C. The mobile phase was composed of 0.1% trifluoroacetic acid in water and acetonitrile. The flow rate was 1 ml/min and detection wavelength was set at 205 nm and 280 nm. Validation was performed in order to demonstrate selectivity, accuracy and precision of the method. Results: The calibration curves showed good linearity (R2 > 0.99). The limits of detection and limits of quantification were within the ranges 0.0159–0.6205 µg/ml and 0.3210–1.8802 µg/ml, respectively. Moreover, the relative standard deviations of intra‑ and inter‑day precision were both <2.98%. The overall recoveries were in the range of 96.23–109.87%. Quantitative analysis of eight compounds in 12 D. superbus samples (D‑1–D‑12) from various regions were analyzed and compared by developed method. Conclusion: As a result, this established method was accurate and sensitive for the quality evaluation of eight compounds isolated from D. superbus and may provide a new basis for quality control of D. superbus.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Yun, B., Yang, H. J., Weon, J. B., Lee, J., Eom, M. R., & Ma, C. J. (2016). Simultaneous Determination of Eight Bioactive Compounds in Dianthus superbus by High‑performance Liquid Chromatography. Pharmacognosy Magazine, 12(46s), S264–S269. https://doi.org/10.4103/0973-1296.182159