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    Purified Essential Oil from Ocimum sanctum Linn. Triggers the Apoptotic Mechanism in Human Breast Cancer Cells

    Thamilvaani Manaharan2,3, Ramaraj Thirugnanasampandan4★, Rajarajeswaran Jayakumar2, M. S. Kanthimathi2, Gunasekar Ramya4, Madhusudhanan Gogul Ramnath4★ Corresponding author

    1. 1Centre of Research for Computational Sciences and Informatics in Biology, Bio-industry, Environment, Agriculture and Healthcare (CRYSTAL).
    2. 2Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
    3. 3Department of Biotechnology, Kongunadu Arts and Science College, GN Mills, Coimbatore, Tamil Nadu, India.

    CORRESPONDENCE

    Ramaraj Thirugnanasampandan

    rtsampandan@yahoo.com

    Received: 09-12-2015; Revised: 02-02-2016.

    Volume 12, Issue 46s · pp. S327–S331 · PUBLISHED 7 July 2016 · DOI: 10.4103/0973-1296.185738

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Essential oil of Ocimum sanctum Linn. exhibited various pharmacological activities including antifungal and antimicrobial activities. In this study, we analyzed the anticancer and apoptosis mechanisms of Ocimum sanctum essential oil (OSEO). Objective: To trigger the apoptosis mechanism in human breast cancer cells using OSEO. Materials and Methods: OSEO was extracted using hydrodistillation of the leaves. Cell proliferation was determined using different concentrations of OSEO. Apoptosis studies were carried out in human breast cancer cells using propidium iodide (PI) and Hoechst staining. Results: We found that OSEO inhibited proliferation (IC50 = 170 μg/ml) of Michigan cancer foundation‑7 (MCF‑7) cells in a dose‑dependent manner. The OSEO also induced apoptosis as evidenced by the increasing number of PI‑stained apoptotic nucleic of MCF‑7 cells. Flow cytometry analysis revealed that treatment with OSEO (50–500 μg/ml) increased the apoptotic cells population (16–84%) dose dependently compared to the control. OSEO has the ability to up‑regulate the apoptotic genes p53 and Bid and as well as elevates the ratio of Bax/Bcl‑2. Conclusion: Our findings indicate that OSEO has the ability as proapoptotic inducer and it could be developed as an anticancer agent.

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      Manaharan, T., Thirugnanasampandan, R., Jayakumar, R., Kanthimathi, M. S., Ramya, G., & Ramnath, M. G. (2016). Purified Essential Oil from Ocimum sanctum Linn. Triggers the Apoptotic Mechanism in Human Breast Cancer Cells. Pharmacognosy Magazine, 12(46s), S327–S331. https://doi.org/10.4103/0973-1296.185738