Phytochemicals and Cytotoxicity of Launaea procumbens on Human Cancer Cell Lines
Preeti Rawat1, Lokesh M. Saroj1, Anil Kumar1, Tryambak D. Singh2, SK. Tewari1, Mahesh Pal1★★ Corresponding author
- 1Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow, India.
- 2Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, U.P, India.
CORRESPONDENCE
Mahesh Pal
Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow, India.
Received: 15-02-2016; Revised: 01-04-2016; Accepted: 01-04-2016.
Volume 12, Issue 47s · pp. 431–5 · PUBLISHED 30 September 2016 · DOI: 10.4103/0973-1296.191452
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ABSTRACT
Background: The plant Launaea procumbens belongs to the family Asteraceae and traditionally used in the treatment rheumatism, kidney, liver dysfunctions and eye diseases. In the present study Phytochemical analysis and fractions of methanolic extract of L. procumbens leaves were tested in vitro for their cytotoxicity. Objectives: Phytochemical analysis and cytotoxic activity of methanolic extract and fractions of Launaea procumbens against four cancer cell lines K562, HeLa, MIA-Pa-Ca-2 and MCF-2 by SRB assay. Materials and Methods: Powdered leaves of Launaea procumbens were extracted sequentially with hexane, ethyl acetate, butanol and water by cold extraction. Phytochemical analysis and cytotoxicity assay were carried out for these fractions using SRB assay against four human cancer cell lines, namely leukemia (K562), cervix (HeLa), pancreatic (MIA-Pa-Ca-2) and breast (MCF-7). Results: Ethyl acetate extract exerts potent cytotoxicity against human leukemia (K562), cervix (HeLa) and breast (MCF-7) cell lines IC50 value of 25.30±0.50, 19.80±0.10 and 36.90±4.90 µg/ ml respectively. Moderately cytotoxic effect found in hexane extract IC50 value of 41±8 and 48.20±0.50 µg/ ml against leukemia (K562), and breast (MCF-7) cancer cell line respectively. The Chemical composition analyzed by GC-MS showed considerable differences in solvent fractions of Launaea procumbens. Conclusion: This study revealed the cytotoxic potential of ethyl acetate and hexane fractions of L.procumbens leaves on different cancer cell lines.
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Rawat, P., Saroj, L. M., Kumar, A., Singh, T. D., Tewari, S., & Pal, M. (2016). Phytochemicals and Cytotoxicity of Launaea procumbens on Human Cancer Cell Lines. Pharmacognosy Magazine, 12(47s), 431–5. https://doi.org/10.4103/0973-1296.191452