Elicitation of Phenolics from the Micropropagated Endangered Medicinal Plant Calligonum polygonoides L. (Polygonoaceae)
Asmaa I. Owis1★, Nada S. Abdelwahab2, Adel A. Abul‑Soad3★ Corresponding author
- 1Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
- 2Department of Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
- 3Horticulture Research Institute, Agricultural Research Center, Cairo, Egypt.
CORRESPONDENCE
Asmaa I. Owis
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
Received: 19-11-2015; Revised: 09-02-2016.
Volume 12, Issue 47s · pp. 465–470 · PUBLISHED 30 September 2016 · DOI: 10.4103/0973-1296.191458
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ABSTRACT
Background: Calligonum polygonoides L. subsp. comosum (L’Hér.) Sosk. is a plant species belonging to family Polygonaceae. Susceptibility to threaten, presence of various chemical constituents, and many medicinal effects reported for this plant in addition to rareness of in vitro culture studies have fuelled the need for its micropropagation and phytochemical investigations of the produced cultures. Objectives: To employ in vitro culture technique for ex situ conservation of C. polygonoides, using the fruit as an explant; establish callus and cell suspension cultures from in vitro germinated plantlets; investigate the production of phenolics through callus, redifferentiated shoot, and cell suspension cultures; attempt to enhance cell capacity to accumulate phenolics using salicylic acid and yeast extract and provide a brief demonstration of biosynthetic pathway leading to phenolic production. Materials and Methods: Modified Murashige and Skoog media supplemented with growth hormones such as kinetin, 1‑naphthaleneacetic acid, 6‑benzylaminopurine, and indole‑3‑acetic acid were used to establish callus, redifferentiated shoots, and cell suspension cultures. Elicitation of cell suspension culture was performed using salicylic acid and yeast extracts. A reversed phase‑high performance liquid chromatography method for determination of phenolic content in the aforementioned cultures was developed. Results: The unorganized callus and cell suspension cultures contained fewer amounts of phenolic compounds than redifferentiated shoots. Elicitation produced massive quantitative reprogramming of phenolic content. Conclusion: The present study offers an alternative and renewable source for this valuable natural plant, provide a chance to improve secondary metabolite yield and serve as a useful tool for studying the biosynthesis of these compounds and its regulation in plant cells.
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Owis, A. I., Abdelwahab, N. S., & Abul‑Soad, A. A. (2016). Elicitation of Phenolics from the Micropropagated Endangered Medicinal Plant Calligonum polygonoides L. (Polygonoaceae). Pharmacognosy Magazine, 12(47s), 465–470. https://doi.org/10.4103/0973-1296.191458