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    In silico Prediction and Wet Lab Validation of Arisaema tortuosum (Wall.) Schott Extracts as Antioxidant and Anti‑breast Cancer Source: A Comparative Study

    Kamal Kant1, Uma Ranjan Lal1, Manik Ghosh1★★ Corresponding author

    1. 1Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India.

    CORRESPONDENCE

    Manik Ghosh

    Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India.

    manik@bitmesra.ac.in

    Received: 03-03-2017; Revised: 25-04-2017.

    Volume 13, Issue 52s · pp. S786–S790 · PUBLISHED 31 January 2018 · DOI: 10.4103/pm.pm_69_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Globally, reactive oxygen species have served as an alarm predecessor toward pathogenesis of copious oxidative stress‑related diseases. The researchers have turned their attention toward plant‑derived herbal goods due to their promising therapeutic applications with minimal side effects. Arisaema tortuosum (Wall.) Schott (ATWS) is used in the traditional medicine since ancient years, but scientific assessments are relatively inadequate and need to be unlocked. Objective: Our aim was designed to validate the ATWS tuber and leaf extracts as an inhibitor of oxidative stress using computational approach. Materials and Methods: The reported chief chemical entities of ATWS were docked using Maestro 9.3 (Schrödinger, LLC, Cambridge, USA) tool and further ATWS extracts (tubers and leaves) were validated with 2,2’‑diphenyl‑1‑picrylhydrazyl (DPPH), 2,2’‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid) diammonium salt (ABTS), ferric‑reducing ability of plasma (FRAP), and sulforhodamine B assays experimentally. Results: In silico results showed notable binding affinity of ATWS phytoconstituents with the receptor (PDB: 3ERT). Experimentally, butanolic tuber fraction confirmed promising antioxidant potential (ABTS: IC50: 271.67 µg/ml; DPPH: IC50: 723.41 µg/ml) with a noteworthy amount of FRAP (195.96 µg/mg), total phenolic content (0.087 µg/mg), and total flavonoid content (7.5 µg/mg) while chloroform fraction (leaves) showed considerable reduction in the cell viability of MCF‑7 cell line. Conclusion: The current findings may act as a precious tool to further unlock novel potential therapeutic agents against oxidative stress.

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      Kant, K., Lal, U. R., & Ghosh, M. (2018). In silico Prediction and Wet Lab Validation of Arisaema tortuosum (Wall.) Schott Extracts as Antioxidant and Anti‑breast Cancer Source: A Comparative Study. Pharmacognosy Magazine, 13(52s), S786–S790. https://doi.org/10.4103/pm.pm_69_17