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    Anticancer Potential of Moringa oleifera Flower Extract in Human Prostate Cancer PC‑3 Cells via Induction of Apoptosis and Downregulation of AKT Pathway

    Jiechang Ju2, Sivapragasam Gothai3, Mohadeseh Hasanpourghadi4, Anmar A. Nasser5, Ibrahim Abdel Aziz Ibrahim6, Naiyer Shahzad6, Ashok Kumar Pandurangan7, Katyakyini Muniandy3, S. Suresh Kumar8, Palanisamy Arulselvan3,9★★ Corresponding author

    1. 1Department of Andrology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou City, China.
    2. 2Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia.
    3. 3Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Departments of, Malaysia.
    4. 4Surgery and.
    5. 5Pharmacology and Toxicology, Faculty of Medicine, Umm Al‑Qura University, Makkah, Saudi Arabia.
    6. 6School of Life Sciences, B.S. Abdur Rahman Crescent University, Chennai.
    7. 7Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Hospital Affiliated to Zhengzhou University, Zhengzhou City, China.
    8. 8Muthayammal Centre for Advanced Research, Muthayammal College of Arts and Science, Rasipuram, Namakkal, Tamil Nadu, India.

    CORRESPONDENCE

    Palanisamy Arulselvan

    Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Departments of, Malaysia.

    arulbio@gmail.com

    Received: 09-11-2017; Revised: 10-01-2018.

    Volume 14, Issue 58 · pp. 477–481 · PUBLISHED 21 November 2018 · DOI: 10.4103/pm.pm_516_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Moringa oleifera (MO), or the horseradish tree, is a pantropical species that is known by such regional names as benzolive, drumstick tree, kelor, marango, mlonge, mulangay, nébéday, saijhan, and sajna. Over the past two decades, many reports have appeared in mainstream scientific journals describing its anticancer properties of MO. While much of this recent enthusiasm indeed appears to be justified, it is critical to evaluate therapeutic activity of MO on prostate cancer to separate rigorous scientific evidence from anecdote. MO contained active polyphenols such as ellagic acid, gallate, methyl gallate, catechol, kaempferol quercetin, and their derivatives. Objective: It is the purpose of this series of brief reviews to critically assess the efficacy of MO flower extract as an antiprostate cancer agent. Materials and Methods: The cell viability of the extract was determined using MTT in different time point and cell cycle progression studies was analyzed by flow cytometry. Various markers of apoptosis were evaluated by immunoblotting. Results: We observed that prostate cancer cells treated with MO flower extract caused 50% inhibition at a dose of 22.61 µg/mL and 6.25 µg/mL in PC‑3 cells at 24 and 48 h, respectively. MO flower extract induced the accumulation of G1 phase cell cycle arrest and apoptosis by annexin V staining. Further, immunoblot detection of PARP cleavage leads to increase the protein expression of caspase‑3 activity and Bax indicates induction of apoptosis. Conclusion: Together, the results suggest for the first time that administration of MO flower extract inhibits prostate cancer progression in PC‑3 cells by interfering AKT pathway.

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      Ju, J., Gothai, S., Hasanpourghadi, M., Nasser, A. A., Ibrahim, I. A. A., Shahzad, N., Pandurangan, A. K., Muniandy, K., Kumar, S. S., & Arulselvan, P. (2018). Anticancer Potential of Moringa oleifera Flower Extract in Human Prostate Cancer PC‑3 Cells via Induction of Apoptosis and Downregulation of AKT Pathway. Pharmacognosy Magazine, 14(58), 477–481. https://doi.org/10.4103/pm.pm_516_17