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    In vitro Evaluation of the Effects of Lycopene on Caspase System and Oxidative DNA Damage in High‑Glucose Condition

    Bunyamin Bazyel1, Semiha Dede2★, Sedat Cetin2, Veysel Yuksek3, Mehmet Taşpinar4★ Corresponding author

    1. 1Health Science High School, Muş Alparslan University, Mus, Turkey.
    2. 2Department of Biochemistry, Veterinary Medical Faculty, Van Yuzuncu Yil University, Turkey.
    3. 3Ozalp Vocational High School, Van Yuzuncu Yil University, Turkey.
    4. 4Department of Medical Biology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey.

    CORRESPONDENCE

    Semiha Dede

    Department of Biochemistry, Veterinary Medical Faculty, Van Yuzuncu Yil University, Turkey.

    sdede@yyu.edu.tr

    Received: 19-09-2018; Revised: 09-11-2018.

    Volume 15, Issue 62s · pp. S30–S33 · PUBLISHED 26 April 2019 · DOI: 10.4103/pm.pm_488_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The present study was planned to investigate the effects of lycopene, on the caspase‑dependent apoptosis in high‑dose glucose (HG)‑treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 μM), L2 (20 μM), and L3 (40 μM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/ thaw method. Caspase 3, 8, 9; 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG); and M30 were determined (enzyme‑linked immunosorbent assay). Results: 8‑OHdG increased in L3 (P ≤ 0.001), whereas L1 caused a decrease in HG group (P ≤ 0.001). Caspase‑3 decreased significantly in L1, L2, and L3G compared to control (P ≤ 0.001) group. Caspase‑8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P ≤ 0.001). There was no difference for Caspase‑9. M30 was not affected by L and HG, which decreased significantly (P ≤ 0.001). Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.

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      Bazyel, B., Dede, S., Cetin, S., Yuksek, V., & Taşpinar, M. (2019). In vitro Evaluation of the Effects of Lycopene on Caspase System and Oxidative DNA Damage in High‑Glucose Condition. Pharmacognosy Magazine, 15(62s), S30–S33. https://doi.org/10.4103/pm.pm_488_18