Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Protective Effects of Ginsenoside Rh1 on Intervertebral Disc Degeneration through Inhibition of Nuclear Factor Kappa‑B Signaling Pathway

    Shuping Zhang1, Tong Wang2, Xuexin Wang3, Yanan Wang3, Peng Wang4, Jinxia Li4, Zhenyu Wang4, Faliang Lin3★★ Corresponding author

    1. 1Department of Clinical Laboratory, Yantai City Hospital for Infectious Diseases, Yantai, China.
    2. 2College of Rehabilitation Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
    3. 3Department of Rehabilitation Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
    4. 4College of Rehabilitation Medicine, Binzhou Medical University, Yantai, China.

    CORRESPONDENCE

    Faliang Lin

    Department of Rehabilitation Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.

    faliang86@outlook.com

    Received: 06-01-2020; Revised: 28-03-2020; Accepted: 22-12-2020.

    Volume 17, Issue 73 · pp. 140–145 · PUBLISHED 15 April 2021 · DOI: 10.4103/pm.pm_579_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: In this study, we aim to explore the protective effect of ginsenoside Rh1 against intervertebral disc degeneration (IDD) and the related mechanism. Materials and Methods: IDD model in Sprague‑Dawley rats was established and the animals were treated with different concentrations of ginsenoside Rh1 for 4 weeks, after this, the animals were sacrificed and the intervertebral disc of was collected for analysis using quantitative polymerase chain reaction. Western blot analysis was performed for quantifying the expression levels of glycosaminoglycans (GAGs) and Types I and Type II collagen. Moreover, serum samples were collected and the expression levels of some of the inflammatory cytokines such as interleukin (IL)‑1 β and IL‑6 were evaluated. Next, we collected the nucleus pulposus (NP) cells from the animals and were divided into five groups: control, IDD, treatment groups with different concentrations of ginsenoside Rh1 (10, 20, and 50 µg/mL). After treatment, the levels of IL‑1 β and IL‑6 in the cell culture supernatant were examined. Then, we performed western blot analysis to quantify the levels of B‑cell lymphoma‑2 (Bcl-2), B‑cell lymphoma‑extra‑large (BCL‑xL), and nuclear factor kappa‑B (NF‑κB) in different groups. Results: We observed that ginsenoside Rh1 significantly downregulated the expression of type I collagen and upregulated the expression of type II collagen and GAG under in vivo conditions. Moreover, the expression levels of IL‑1 β and IL‑6 in the serum samples of IDD rats and cell culture supernatant of NP cells isolated from the IDD rats were significantly increased. However, ginsenoside Rh1 significantly increased the levels of Bcl‑2 and Bcl‑xL and decreased the levels NF‑κB both under in vitro and in vivo conditions. Conclusion: Ginsenoside Rh1 demonstrated protective effect against the IDD via regulation of IL‑1 β/NF‑κB signaling pathway.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Zhang, S., Wang, T., Wang, X., Wang, Y., Wang, P., Li, J., Wang, Z., & Lin, F. (2021). Protective Effects of Ginsenoside Rh1 on Intervertebral Disc Degeneration through Inhibition of Nuclear Factor Kappa‑B Signaling Pathway. Pharmacognosy Magazine, 17(73), 140–145. https://doi.org/10.4103/pm.pm_579_19