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    Article

    Preparation and Anti‑fatigue Effects of Vicatia thibertica Polysaccharide

    Weiwei Zhang1, Jincheng Duan2, Chunyan Ji1, Zhengchun He3, Guirong Shi3, Meixian Guo1★, Xiaobo Liu1★★ Corresponding author

    1. 1Department of Pharmacology, College of Pharmacy, Dali University, Dali City, Dali Bai Autonomous Prefecture, Yunnan Province, China.
    2. 2Huidong County Maternal and Child Health and Family Planning Service Center, Huidong, Guangdong, China.
    3. 3Department of Medicinal Chemistry, College of Pharmacy, Dali University, Dali City, Dali Bai Autonomous Prefecture, Yunnan Province, China.

    CORRESPONDENCE

    Meixian Guo

    Department of Pharmacology, College of Pharmacy, Dali University, Dali City, Dali Bai Autonomous Prefecture, Yunnan Province, China.

    yndllyo@126.com

    Received: 13-05-2021; Accepted: 09-09-2021.

    Volume 18, Issue 77 · pp. 133–142 · PUBLISHED 28 March 2022 · DOI: 10.4103/pm.pm_213_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: We intended to purify and gauge the anti‑fatigue effects of a polysaccharide isolated from Vicatia thibertica polysaccharide 1 (VTP1). Materials and Methods: First, the exercise fatigue model was recognized in Kunming mice. After 14 days of continuous intragastric administration of VTP1 (50, 100, and 200 mg/kg/day), we assessed the anti‑fatigue and antioxidant effects of VTP1 on fatigued mice. In addition, the effects of VTP1 on mitochondrial morphology of skeletal muscle of fatigue mice were detected by transmission electron microscopy. Meanwhile, the expression of mitochondrial DNA was noticed. Finally, using Western blot, immunohistochemistry, and real‑time quantitative polymerase chain reaction, respectively, to sense the expression of sirttuin1, peroxisome proliferative activated receptor‑γ coactivator 1α (PGC‑1α), and nuclear respiratory factor 1 (NRF1). Results: VTP1 meaningly extends the time to exhaustion during weight‑bearing swimming and improves the endurance and bodyweight of fatigued mice. Furthermore, VTP1 exerts antioxidant effects, reduces creatine kinase, blood urea nitrogen and lactate dehydrogenase in the serum, surges superoxide dismutase and cuts malondialdehyde in the liver. Meanwhile, VTP1 diminishes degeneration of mitochondria in the skeletal muscle of mice with exercise fatigue and improves the biosynthetic efficiency of mitochondria in skeletal muscle. Finally, VTP1 can upregulate the mRNA and protein expression of PGC‑1α and NRF1. Conclusion: VTP1 has an anti‑fatigue effect, and the effect may be mediated by the PGC‑1α/NRF1 signaling pathway.

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      Zhang, W., Duan, J., Ji, C., He, Z., Shi, G., Guo, M., & Liu, X. (2022). Preparation and Anti‑fatigue Effects of Vicatia thibertica Polysaccharide. Pharmacognosy Magazine, 18(77), 133–142. https://doi.org/10.4103/pm.pm_213_21