Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Anticarcinogenic Effect of Brucine on DMBA‑Induced Skin Cancer via Regulation of PI3K/AKT Signaling Pathway

    Jie Wang1, Zhenxing He1,2, Juhua Zhao1, Wenming Xiao3, Lingling Xiong1, Wei Chen4★★ Corresponding author

    1. 1Departments of Dermatology, Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong 637000, Sichuan, China.
    2. 2Hepatobiliary Surgery, Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong 637000, Sichuan, China.
    3. 3Chongqing Vcham Plastic Surgery Hospital Ltd, Jiangbei district Chongqing 400020, Chongqing, China.
    4. 4Department of Pathophysiology, Basic Medical School, North Sichuan Medical College, Nanchong 637500, Sichuan, China.

    CORRESPONDENCE

    Wei Chen

    Department of Pathophysiology, Basic Medical School, North Sichuan Medical College, Nanchong 637500, Sichuan, China.

    chengwei197959@163.com

    Received: 06-04-2021; Revised: 31-07-2021; Accepted: 14-10-2021.

    Volume 18, Issue 77 · pp. 29–35 · PUBLISHED 28 March 2022 · DOI: 10.4103/pm.pm_152_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The main intention of these hypothesis anticancer effects of brucine on 7,12‑dimethylbenz(a)anthracene (DMBA)‑induced skin cancer in mouse model through phosphatidylinositol 3‑kinase/protein kinase B (PI3K/AKT) regulating signaling pathway via the suppressive effect on cell proliferation and apoptotic pathways in mouse model. Materials and Methods: Brucine action on DMBA‑induced mouse skin body weight, tumor volume, histology, biochemical, molecular marker analysis using spectrophotometric, Western blotting, and real‑time polymerase chain reaction analysis. Results: Brucine stifled the lipid peroxidation (TBARS), suggestively augmented the levels of antioxidants (superoxide dismutase, catalase, glutathione peroxidase, and glutathione), and brought back the status of xenobiotic enzymes (Cyt‑p450, Cyt‑b5, and glutathione S‑transferases). In brucine administered since skin tissues presented the cell proliferative protein marker expression of PI3K, and AKT were downregulated compared to the DMBA‑applied skin tumor tissues protein. Further, brucine has downregulated the proliferating cell nuclear antigen, cyclin‑D1, and p53 expressions. In the apoptotic expression, markers such as Bcl‑2, Bax, caspase‑3, and caspase‑9 were upregulated compared to the DMBA‑induced skin cancer. From these data, we diseased and brucine potential to suppress the proliferative cell markers induces apoptotic expressions. Conclusion: The current search settled that administering brucine chemopreventive and chemotherapeutic effect means for the cancer management in clinical tactic.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Wang, J., He, Z., Zhao, J., Xiao, W., Xiong, L., & Chen, W. (2022). Anticarcinogenic Effect of Brucine on DMBA‑Induced Skin Cancer via Regulation of PI3K/AKT Signaling Pathway. Pharmacognosy Magazine, 18(77), 29–35. https://doi.org/10.4103/pm.pm_152_21