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    Article

    Rehmanniae Radix‑Induced Apoptosis via Inhibition of PI3K/ AKT/mTOR Signaling Pathways in Human Hepatocellular Carcinoma Cell Lines SMMC‑7721

    Bonian Liao1, Fangli Luo1, Shu Zhang1, Zhengwan Deng1, Lin Cai1★★ Corresponding author

    1. 1Department of Traditional Chinese Medicine, Affiliated Hospital of North Sichuan Medical College, Shunqing District, Nanchong, Sichuan, China.

    CORRESPONDENCE

    Lin Cai

    Department of Traditional Chinese Medicine, Affiliated Hospital of North Sichuan Medical College, Shunqing District, Nanchong, Sichuan, China.

    zyjcjys@163.com

    Received: 04-04-2021; Revised: 19-09-2021; Accepted: 14-10-2021.

    Volume 18, Issue 77 · pp. 4–9 · PUBLISHED 28 March 2022 · DOI: 10.4103/pm.pm_147_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: To inspect RR leaves extracts pro‑apoptotic activities on SMMC‑7721 liver cancer cell lines. Materials and Methods: Anti‑cancer effect of RR extracts in SMMC‑7721 liver cancer cell lines was aged rate of cell apoptosis (AO/EB, 4′,6‑diamidino‑2‑phenylindole [DAPI], and PI staining), ΔΨm (Rh‑123 staining), intracellular Reactive oxygen species (ROS) (DCFH‑DA staining), quantitative polymerase chain reaction (mRNA expressions of Bax, p53, Bcl‑2, caspase‑3,‑9,‑8, IL‑6, PI3K/AKT, and mTOR). Results: It has been shown that RR leaf ethanolic extracts suggestively declined cell viability, augmented the rate of cell apoptosis (AO/EB, DAPI, and PI staining), and caused the potential for ΔΨm, and induced over‑accumulation of intracellular ROS (DCFH‑DA staining) in SMMC‑7721 cells after 24 h of exposure. In SMMC‑7721 cell lines, we have examined anti‑cancer activity to scrutinize the underlying mechanism by assessing the mRNA expressions of Bax, p53, Bcl‑2, caspase‑3,‑9,‑8, IL‑6, PI3K/AKT, and mTOR. Results displayed the significant inhibition of the apoptosis as stated above related proteins. Conclusion: RR extracts exhibited its potential anti‑cancer activity against SMMC‑7721 cells by prompting apoptosis‑induced cell death and inhibiting PI3K/AKT/mTOR signaling.

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      Liao, B., Luo, F., Zhang, S., Deng, Z., & Cai, L. (2022). Rehmanniae Radix‑Induced Apoptosis via Inhibition of PI3K/ AKT/mTOR Signaling Pathways in Human Hepatocellular Carcinoma Cell Lines SMMC‑7721. Pharmacognosy Magazine, 18(77), 4–9. https://doi.org/10.4103/pm.pm_147_21