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    Comparative Study on the Antitumour Activity of 20(R)‑ginsenoside Rh2 and 20(S)‑ginsenoside Rh2 from Panax ginseng against Non‑small Cell lung Cancer in vitro

    Xinze Liu2, Xin Jin3, Xinmin Wu4, Lin Feng2, Hongmei Yang2, KaiJing Sun2, Xue Yang5, Ruina Lv6, Yuhe Ren7, Mengle Xie3, Jianhua Lv6, Lan Yao6, Daiyao Liu6, Wei Wu2, Shuying Liu2, Changbao Chen2★, Xilin Wan2,6★★ Corresponding author

    1. 1Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China.
    2. 2Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, China.
    3. 3Department of Neurosurgery, First Hospital of Jilin University, Changchun, China.
    4. 4Cardiovascular Medicine and Cardiac Rehabilitation Center, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, China.
    5. 5International Cooperation Research Center of China for New Germplasm and Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun, China.
    6. 6Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, China.

    CORRESPONDENCE

    Changbao Chen

    Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, China.

    ccb2021@126.com

    Received: 19-12-2021; Revised: 03-04-2022; Accepted: 01-08-2022.

    Volume 18, Issue 80 · pp. 1011–1024 · PUBLISHED 23 November 2022 · DOI: 10.4103/pm.pm_583_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Non‑small cell lung cancer (NSCLC) is often harmful to human health. It is common in people who smoke or smoke passively, and the affected people are older and find it difficult to recover. The main treatment methods are surgery, radiotherapy and chemotherapy. This paper aims to study the effects of different configurations of ginsenoside Rh2 on NSCLC 95D and NCI‑H460 cells. To provide clinical value for the treatment of NSCLC. Materials and Methods: CCK‑8 was used to determine the proliferation of 95D and NCI‑H460 cells with different concentrations of ginsenoside Rh2 (0, 0.05, 0.1 and 0.2 mg/mL), and IC50 was calculated. Cell cycle and apoptosis were detected by immunofluorescence staining and flow cytometry. Results: 20(R)‑ginsenoside‑Rh2 and 20(S)‑ginsenoside‑Rh2 inhibited the proliferation and colony formation of NSCLC 95D and NCI‑H460 cells induced cell cycle arrest in a time and dose‑dependent manner in G1/S and promote apoptosis. Conclusion: Ginsenoside Rh2 induces antitumour activity by inhibiting proliferation and colony formation of 95D and NCI‑H460 cells and inducing cell cycle arrest and apoptosis. Comparing the two configurations of ginsenoside Rh2, the effect of 20(R)‑ginsenoside‑Rh2 is stronger.

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      Liu, X., Jin, X., Wu, X., Feng, L., Yang, H., Sun, K., Yang, X., Lv, R., Ren, Y., Xie, M., Lv, J., Yao, L., Liu, D., Wu, W., Liu, S., Chen, C., & Wan, X. (2022). Comparative Study on the Antitumour Activity of 20(R)‑ginsenoside Rh2 and 20(S)‑ginsenoside Rh2 from Panax ginseng against Non‑small Cell lung Cancer in vitro. Pharmacognosy Magazine, 18(80), 1011–1024. https://doi.org/10.4103/pm.pm_583_21