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    Article

    Ethanolic extract of Allium sativum has antiproliferative effect on Hep2 and L929 cell lines

    Jalil Tavakkol Afshari1★, Moosa-Alreza Hajzadeh2, Ahmad Ghorbani3, Heydar Parsaie4★ Corresponding author

    1. 1Immunology and cell culture laboratory, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Islamic Republic of Iran.
    2. 2Department of Physiology, Mashhad University of Medical Sciences, Islamic Republic of Iran.
    3. 3Department of Physiology, Mashhad University of Medical Sciences, Islamic Republic of Iran.
    4. 4Department of Pharmacology, Mashhad University of Medical Sciences, Islamic Republic of Iran.

    CORRESPONDENCE

    Jalil Tavakkol Afshari

    Immunology and cell culture laboratory, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Islamic Republic of Iran.

    ghorbani_ahmad@yahoo.com

    Volume 2, Issue 5 · pp. 31–32 · PUBLISHED 2006 · DOI:

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Cancer of the larynx represents worldwide approximately 1-2% of all cancers and generally occurs predominantly in males. Experimentally, Allium sativum and its associated sulfur components are reported to suppress tumor incidence in breast, colon, skin, uterine, esophagus and lung. In this study, we investigated in vitro effect of ethanolic extract of A. sativum on human larynx tumor cell (Hep2) and non-tumor cell (L929) lines. The cells were cultivated and incubated with different concentrations (0 (control), 0.5, 1, 2, 4, 8 and 12 mg/ml) of the whole extract. After 24h, morphological changes in both cells showed growth inhibitory effects at concentrations of 4, 8 and 12 mg/ml of the extract. These changes became more prominent on the second and third day. MTT assay results indicated a significant difference between Hep2 and L929 cells incubated in all concentrations and the cells incubated in the absence of the extract in reduction of percentage of surviving cells. The results of this study demonstrate that this type of A. sativum extract exert anti-proliferative effect on both Hep2 and L929 cell lines.

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      Afshari, J. T., Hajzadeh, M., Ghorbani, A., & Parsaie, H. (2006). Ethanolic extract of Allium sativum has antiproliferative effect on Hep2 and L929 cell lines. Pharmacognosy Magazine, 2(5), 31–32.