Resatovir Combined with Glycyrrhizic Acid Ameliorates Osteoarthritis and Enhances Autophagy in Chondrocytes
Qijie Mei1, Wenfei Xu1, Qi Hu2, Jinrong Guo1, Chao Zeng1, Chunyu Ming3, Chongming Li2, Changshen Yuan1, Kan Duan1★★ Corresponding author
- 1Department of Orthopedics and Injuries of the Extremities, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi, CHINA.
- 2Graduate School of Guangxi University of Chinese Medicine, Nanning, Guangxi, CHINA.
- 3Department of Geriatrics, Ruikang Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, CHINA.
CORRESPONDENCE
Kan Duan
Department of Orthopedics and Injuries of the Extremities, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi, CHINA.
Received: 07-03-2024; Accepted: 18-07-2024.
Volume 21, Issue 1 · pp. 133–141 · PUBLISHED 2025 · DOI: 10.1177/09731296241283126
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Osteoarthritis (OA) is a chronic disease worldwide. With resatovir usually used as an inhibitor, resatovir and glycyrrhizic acid have several pharmacological activities. Objectives: We intend to explore the mechanism underlying resatovir combined with glycyrrhizic acid in OA. Materials and Methods: After the establishment of an animal model of OA through anterior cruciate ligament transection and destabilization of the medial meniscus, the rats were subjected to intramuscular injection of resatovir combined with glycyrrhizic acid (60 mg/kg) or 5% glucose (60 mg/kg) every day for 4 weeks. During the progress, the rat movement and the bend score of knees were observed through behavioral studies. Additionally, the SKP2 gene expression and toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB)-related signaling pathways were examined, as apoptosis and autophagy were evaluated by flow cytometry. Results: With increased inflammatory cells in the model group, treatment with resatovir combined with glycyrrhizic acid significantly decreased the number of inflammatory cells. Meanwhile, the treatment resulted in decreased SKP2 expression in OA cells when hindering the migration of preosteoclast cells. Interestingly, it was observed that the bend score increased over the treatment with resatovir plus glycyrrhizic acid and TIPPI% declined dramatically. Resatovir treatment enhanced autophagy and apoptosis in OA cells and decreased pP65, P65, and LC3 expression. Conclusion: Resatovir combined with glycyrrhizic acid can inhibit the inflammatory response through decreasing SKP2 expression and induce autophagy activation in OA cells through TLR4/NF-κB signaling, thereby attenuating OA progression.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Mei, Q., Xu, W., Hu, Q., Guo, J., Zeng, C., Ming, C., Li, C., Yuan, C., & Duan, K. (2025). Resatovir Combined with Glycyrrhizic Acid Ameliorates Osteoarthritis and Enhances Autophagy in Chondrocytes. Pharmacognosy Magazine, 21(1), 133–141. https://doi.org/10.1177/09731296241283126