Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Determination of 5-hydroxymethyl-2-furaldehyde of crude and processed Fructus Corni in freely moving rats using in vivo microdialysis sampling and liquid chromatography

    Enhong Ouyang1★, Chengrong Zhang2, Xiaomeng Li2★ Corresponding author

    1. 1Department of Pharmaceutics, Shaoyang Medical College, Shangyang 422000, China.
    2. 2Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 310053, PR, China.

    CORRESPONDENCE

    Enhong Ouyang

    Department of Pharmaceutics, Shaoyang Medical College, Shangyang 422000, China.

    ouyangenhong1975@163.com

    Received: 25-06-2011; Revised: 04-09-2011.

    Volume 7, Issue 28 · pp. 271–276 · PUBLISHED 30 November 2011 · DOI: 10.4103/0973-1296.90394

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Fructus Corni is derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc. 5-hydroxymethyl-2-furaldehyde (5-HMF) is an important active composition of the Fructus Corni. However, there have been no reports on the concentration of 5-HMF in freely moving rats using microdialysis coupled with HPLC. Materials and Methods: The concentration of 5-HMF in free-moving rats after intra-gastric (i.g.) administration of the water extract of Fructus Corni and JZP was analyzed by microdialysis coupled with high-performance liquid chromatographic (HPLC). Results: Results demonstrated that the concentration of 5-HMF in microdialysate was 1.4951 μg/l, but higher in rat microdialysate after i.g. administration of the aqueous extract of JZP (5.2662 μg/l). Conclusion: This method is proved to be rapid, accurate and simple. Real-time in vivo monitoring the concentration of 5-HMF provides the theoretical basis for further explaining the processing mechanism of Fructus Corni.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Ouyang, E., Zhang, C., & Li, X. (2011). Determination of 5-hydroxymethyl-2-furaldehyde of crude and processed Fructus Corni in freely moving rats using in vivo microdialysis sampling and liquid chromatography. Pharmacognosy Magazine, 7(28), 271–276. https://doi.org/10.4103/0973-1296.90394