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    Article

    Simultaneous determination of four bioactive compounds in Verbena officinalis L. by using highperformance liquid chromatography

    Zhen Liu1★, Zhiwei Xu1, Hanyu Zhou1, Gang Cao1, Xiao-Dong Cong1, Yun Zhang1, Bao-Chang Cai2,3,4★ Corresponding author

    1. 1Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China.
    2. 2Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China.
    3. 3National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing, People’s Republic of, China.

    CORRESPONDENCE

    Zhen Liu

    Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China.

    Received: 21-10-2011; Revised: 08-11-2011.

    Volume 8, Issue 30 · pp. 162–165 · PUBLISHED 23 May 2012 · DOI: 10.4103/0973-1296.96575

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Verbena officinalis L., called mabiancao in Chinese, is derived from the aerial part of Herba Verbanae. It is a traditional Chinese medicine commonly used in China and northern Europe, which is widely used for clearing away heat and detoxicating, promoting blood circulation, and removing blood stasis. This paper describes a sensitive and specific assay for the determination of four bioactive compounds in V. officinalis L. Materials and Methods: In this paper, the four components were separated on an Agilent Zorbax Extend C18 column (250 mm × 4.6 mm × 5 μm) and detected by a diode array detector. The mobile phase was composed of (a) aqueous phosphoric acid (0.1%, v/v) and (b) acetonitrile using a gradient elution. Analytes were performed at 30ºC with a flow rate of 1.0 ml/min and UV detection at 203, 238, and 331 nm. Results: All calibration curves showed good linear regression (r2 ≥ 0.9999) within tested ranges. Overall intra- and interday variations were less than 1.84%, and the average recoveries were 97.32–102.81% for analytes. Discussion and Conclusion: The proposed method would be sensitive enough and reliable for comprehensive quality control for clinical use and modernization of V. officinalis L.

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      Liu, Z., Xu, Z., Zhou, H., Cao, G., Cong, X., Zhang, Y., & Cai, B. (2012). Simultaneous determination of four bioactive compounds in Verbena officinalis L. by using highperformance liquid chromatography. Pharmacognosy Magazine, 8(30), 162–165. https://doi.org/10.4103/0973-1296.96575