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    Simultaneous determination of eleven characteristic lignans in Schisandra chinensis by high-performance liquid chromatography

    Junyang Hu2, Chunqin Mao2, Xiaodong Gong2, Tulin Lu2,3★, Han Chen2, Zhijun Huang4, Baochang Cai3★ Corresponding author

    1. 1College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046, PR China.
    2. 2Jiangsu Province Key Research Laboratory of Chinese Medicine Processing, Nanjing, 210046, PR China.
    3. 3Jiangsu Province Key Laboratory of Acupuncture of Nanjing University of Chinese Medicine, Nanjing, 210046, PR, China.

    CORRESPONDENCE

    Tulin Lu

    Jiangsu Province Key Research Laboratory of Chinese Medicine Processing, Nanjing, 210046, PR China.

    lutuling2005@126.com

    Received: 19-02-2012; Revised: 28-03-2012.

    Volume 9, Issue 34 · pp. 155–161 · PUBLISHED 30 April 2013 · DOI: 10.4103/0973-1296.111280

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Schisandra chinensis, one of the well-known traditional Chinese herbal medicines, is derived from the dry ripe fruits of Schisandra chinensis (Turcz.) Baill. according to the 9th China Pharmacopeia. Lignans are the main components isolated from extracts of S. chinensis and their content varies depending on where S. chinensis was collected. We have established a qualitative and quantitative method based on the bioactive lignans for control of the quality of S. chinensis from different sources. Materials and Methods: To develop a high-performance liquid chromatography method, an Elite ODS C18 column (250 mm × 4.6 mm, 5μm) at a column temperature of 30°C and flow rate of 1.0ml/min using acetonitrile (A) and water (B) as the mobile phase with a linear gradient and the peaks were monitored at 217 nm. Results: All calibration curves showed good linearity (r ≥ 0.9995) within test ranges. This method showed good repeatability for the quantification of these eleven components in S. chinensis with intra- and inter-day relative standard deviations less than 0.43% and 1.21%, respectively. In the recovery test, results of accuracy ranged from 99.51% to 101.31% with RSD values less than 2.68%, Conclusion: The validated method can be successfully applied to quantify the eleven investigated components in 22 samples of S. chinensis from different sources.

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      Hu, J., Mao, C., Gong, X., Lu, T., Chen, H., Huang, Z., & Cai, B. (2013). Simultaneous determination of eleven characteristic lignans in Schisandra chinensis by high-performance liquid chromatography. Pharmacognosy Magazine, 9(34), 155–161. https://doi.org/10.4103/0973-1296.111280