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    Article

    Ultra-performance liquid chromatography fi ngerprinting for quality control of Phragmitis rhizoma (Lugen) produced in Baiyangdian

    Hong Li1, Yu-Mei Gao2, Jing Zhang1, Lin Wang1★, Xiao-Xin Wang1★ Corresponding author

    1. 1Department of Pharmaceutical Engineering, Hebei Chemical and Pharmaceutical College, Shijiazhuang, China.
    2. 2Medical College, Hebei University of Engineering, Handan, Hebei, China.

    CORRESPONDENCE

    Lin Wang

    Department of Pharmaceutical Engineering, Hebei Chemical and Pharmaceutical College, Shijiazhuang, China.

    wanglin8457@yahoo.com.cn

    Received: 31-08-2012; Revised: 19-09-2012.

    Volume 9, Issue 36 · pp. 285–289 · PUBLISHED 7 September 2013 · DOI: 10.4103/0973-1296.117810

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: To establish an ultra-performance liquid chromatography (UPLC) fi ngerprinting method for quality control of Phragmitis rhizoma from Baiyangdian. Materials and Methods: Ultrasonic extraction with 70% methanol was performed on 10 samples of P. rhizoma collected from 10 different villages in Baiyangdian. The sample solutions were analyzed by Waters UPLC equipped with the ACQUITY UPLC BEH C18 column and photodiode array (PDA) detector, and gradient eluted with acetonitrile/water as the mobile phase. The fl ow rate was set to 0.1 mL/min; the column temperature was set to 25°C; and the detection wavelength was set to 285 nm. Results: The chromatograms of the 10 samples showed 27 common peaks, of which one was identifi ed as the ferulic acid standard. The similarity indexes were all above 0.82. Hierarchical cluster analysis showed that the constituents and their quantities differed according to the diameter of the original plant, which is related to its age. Conclusion: The UPLC fi ngerprinting method had the advantages of being fast, accurate, and highly effi cient; this indicated that it can be used for quality control of P. rhizoma produced in Baiyangdian. Also, the relation between the quality and diameter/age of the plant needs to be further investigated.

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      Li, H., Gao, Y., Zhang, J., Wang, L., & Wang, X. (2013). Ultra-performance liquid chromatography fi ngerprinting for quality control of Phragmitis rhizoma (Lugen) produced in Baiyangdian. Pharmacognosy Magazine, 9(36), 285–289. https://doi.org/10.4103/0973-1296.117810