Protective effects of apigenin against methyl methanesulfonate induced hsp70 expression in the third instar larvae of transgenic Drosophila melanogaster (hsp70- lacZ)Bg 9
Yasir Hasan Siddique1★, Mohammad Afzal2★ Corresponding author
- 1Drosophila Transgenics Laboratory, India.
- 2Human Genetics and Toxicology Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
CORRESPONDENCE
Yasir Hasan Siddique
Drosophila Transgenics Laboratory, India.
Volume 3, Issue 2 · pp. · PUBLISHED 2012 · DOI: 10.4103/0976-500X.95525
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ABSTRACT
A transgenic Drosophila melanogaster line expressing bacterial b-galactosidase as a response to stress was used in this study.[6] The flies and larvae were cultured on standard Drosophila food containing agar, cornmeal, sugar, and yeast at 24°C. MMS at 0.5 and 1.0 ml/ml of food concentration alone and along with 0.1, 0.5, and 1.0 ml/ml of apigenin were established. The third instar larvae were allowed to feed on them for different time intervals (12, 24, and 48 h). For quantifying the β-galactosidase activity, the method as described by Nazir et al. was followed.[6] The extent of tissue damage in larvae exposed to different concentrations of MMS alone and along with apigenin was assayed by a dye exclusion test.[6] Statistical analysis was carried out by Student’s ‘t’-test. P<0.05 was considered statistically significant.
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Siddique, Y. H., & Afzal, M. (2012). Protective effects of apigenin against methyl methanesulfonate induced hsp70 expression in the third instar larvae of transgenic Drosophila melanogaster (hsp70- lacZ)Bg 9. Journal of Pharmacology and Pharmacotherapeutics, 3(2). https://doi.org/10.4103/0976-500X.95525