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    Journal of Pharmacology and PharmacotherapeuticsNOW IN
    Article

    Vasorelaxation of goat mesenteric artery is mediated by endothelial Na+‑K+‑ATPase

    R. Sathiskumar1★, Bimal Prasanna Mohanty2, Subas Chandra Parija1★ Corresponding author

    1. 1Department of Pharmacology and Toxicology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India.
    2. 2FREM Division, Central Inland Fisheries Research Institute, ICAR, Kolkata, West Bengal, India.

    CORRESPONDENCE

    R. Sathiskumar

    Department of Pharmacology and Toxicology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India.

    Received: 06-03-2015; Revised: 09-08-2015; Accepted: 09-09-2015.

    Volume 6, Issue 4 · pp. 204–210 · PUBLISHED 2015 · DOI: 10.4103/0976-500X.171884

    View on J Pharmacol. Pharmacother. original site ↗

    ABSTRACT

    Objective: To examine the role of Na+‑K+‑ATPase and K+ channels in mediating vasorelaxation in the superior mesenteric artery of Capra hircus. Materials and Methods: Goat superior mesenteric artery (GSMA) was cut into 1.5–2 mm circular rings and mounted in a thermostatically controlled (37°C ± 0.5°C) organ bath containing 20 ml of modified Krebs‑Henseleit saline (MKHS) (pH 7.4), with continuous aeration under 1.5 g tension for 90 min. Endothelium‑intact (ED+) or endothelium‑denuded (ED−) GSMA ring was contracted with phenylephrine (PE) or 5‑hydroxytryptamine (5‑HT) (1 μM–0.1 mM) in the absence or presence of ouabain (0.1 µM). KCl (1 μM–10 mM) was added cumulatively to K+‑free MKHS‑pre‑contracted (ED+/−) rings in the absence or presence of ouabain (0.1 μM) or barium (1 μM) or 4‑aminopyridine (1 μM). Results: Ouabain did not alter the basal tone of the arterial ring. The contractile response induced by PE (Emax: 50.46 ± 2.68, pD2: 5.53 ± 0.04) and 5‑HT (Emax: 30.86 ± 1.33, pD2: 6.17 ± 0.03) in ED+ ring was significantly (P < 0.001) augmented in ED− rings (PE: Emax: 93.30 ± 2.11, pD2: 6.41 ± 0.04; 5‑HT: Emax: 95.07 ± 0.99, pD2: 6.27 ± 0.03). The contractile response induced by PE and 5‑HT in ED+ or ED− rings in the presence of ouabain was almost identical with that of ED− rings. Vasorelaxation of KCl (Emax: 2.90 ± 1.14, pD2: 3.9 ± 0.03) was significantly attenuated in the presence of ouabain (Emax: 73.8 ± 5.16, pD2: 4.3 ± 0.04), Ba2+ (Emax: 16.34 ± 4.7, pD2: 3.22 ± 0.02), 4‑AP (Emax: 18.16 ± 2.4, pD2: 3.68 ± 0.03), ouabain and Ba2+ (Emax: 70.09 ± 3.66, pD2: 4.41 ± 0.04), and ouabain and 4‑AP (Emax: 66.98 ± 4.61, pD2: 4.13 ± 0.06). Conclusion: The vasorelaxation in GSMA is mediated by the endothelium‑derived hyperpolarizing factor (EDHFs) such as ouabain‑sensitive Na+‑K+‑ATPase, KIR and Kv channels.

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      Sathiskumar, R., Mohanty, B. P., & Parija, S. C. (2015). Vasorelaxation of goat mesenteric artery is mediated by endothelial Na+‑K+‑ATPase. Journal of Pharmacology and Pharmacotherapeutics, 6(4), 204–210. https://doi.org/10.4103/0976-500X.171884