Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Fraction and chemical analysis of antioxidant active polysaccharide isolated from fl ue-cured tobacco leaves

    Chunping Xu1★, Chenchen Yang1, Duobin Mao1★ Corresponding author

    1. 1Department of Tobacco Engineering, College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R, China.

    CORRESPONDENCE

    Chunping Xu

    Department of Tobacco Engineering, College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R, China.

    c.p.xu@zzuli.edu.cn

    Received: 07-07-2013; Revised: 24-07-2013.

    Volume 10, Issue 37 · pp. 66–69 · PUBLISHED 7 February 2014 · DOI: 10.4103/0973-1296.126664

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The metabolic compounds from tobacco were investigated to posses various biological activities, such as antioxidant, antimicrobial and neuroprotective activities. Materials and Methods: Polysaccharides were isolated from the ultrasound-assisted extraction of fl ue-cured tobacco with hot water. The purifi ed polysaccharides were analyzed by GC-MS, FT-IR, and TGA (Thermogravimetric analysis). The antioxidant activities of purifi ed polysaccharides were evaluated in vitro. Results: Two polysaccharides (Fr-I and Fr-II) were isolated and purifi ed. The analysis of monosaccharide composition in the polysaccharide by GC/MS revealed that Fr-I and Fr-II were heteropolysaccharides. Fr-I consisted of D-mannose and galactose, ribose and arabinose, while Fr-II was mainly composed of glucose, allose, galactose and mannose. Thermo gravimetric analysis (TGA) indicated that the degradation temperature (Td) of the Fr-I (241 °C) was higher than that of Fr-II (220 °C). Both samples showed inhibitory effects on OH (hydroxyl) and DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate) radical in a concentration-dependent manner. Comparing Fr-I with Fr-II, the latter has a strong scavenging ability. Conclusion: Both polysaccharide fractions showed signifi cant antioxidant effects. Various factors infl uenced the antioxidant activity of polysaccharides.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Xu, C., Yang, C., & Mao, D. (2014). Fraction and chemical analysis of antioxidant active polysaccharide isolated from fl ue-cured tobacco leaves. Pharmacognosy Magazine, 10(37), 66–69. https://doi.org/10.4103/0973-1296.126664