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    A validated high performance liquid chromatograph-photodiode array method for simultaneous determination of 10 bioactive components in compound hongdoushan capsule

    Liancai Zhu1, Xian Yang2, Jun Tan3, Bochu Wang1★, Xue Zhang4★ Corresponding author

    1. 1Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing, China.
    2. 2College of Life Sciences, Chongqing Normal University, Chongqing, China.
    3. 3Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, China.
    4. 4National Center of Biomedical Analysis, Chongqing Academy of Chinese Mater ia Medica, Chongqing, China.

    CORRESPONDENCE

    Bochu Wang

    Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing, China.

    zhuliancai75@126.com

    Received: 20-12-2012; Revised: 15-02-2013.

    Volume 10, Issue 37 · pp. 83–88 · PUBLISHED 7 February 2014 · DOI: 10.4103/0973-1296.126673

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The compound Hongdoushan capsule (CHC) is widely known as compound herbal preparation and is often used to treat ovarian cancer and breast cancer, and to enhance the body immunity, etc., in clinical practice. Objective: To determine simultaneously 10 bioactive components from CHC, namely glycyrrhetinic acid, liquiritin, glycyrrhizin, baccatin III, 10- deacetylbaccatin III, cephalomannine, taxol, ginsenoside Rg1, ginsenoside Re, and ginsenoside Rb1. Materials and Methods: A high performance liquid chromatograph method coupled with photodiode array detector was developed and validated for the 1st time. Chromatographic analysis was performed on a SHIMADZU C18 by utilizing a gradient elution program. The mobile phase was acetonitrile (A)-water (B) at a fl ow rate of 0.8 mL/min. Results: The calibration curve was linear over the investigated concentration ranges with the values of r2 higher than 0.9993 for all the 10 bioactive components. The average recovery rates range from 98.4% to 100.5% with relative standard deviations ≤2.9%. The developed method was successfully applied to analyze 10 compounds in six CHC samples from different batches. In addition, the herbal sources of 32 chromatographic peaks were identifi ed through comparative studying on chromatograms of standard, the respective extracts of Hongdoushan, RenShen, GanCao, and CHC. Conclusion: All the results imply that the accurate and reproducible method developed has high separation rate and enables the determination of 10 bioactive components in a single run for the quality control of CHC.

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      Zhu, L., Yang, X., Tan, J., Wang, B., & Zhang, X. (2014). A validated high performance liquid chromatograph-photodiode array method for simultaneous determination of 10 bioactive components in compound hongdoushan capsule. Pharmacognosy Magazine, 10(37), 83–88. https://doi.org/10.4103/0973-1296.126673