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    Article

    Leucas aspera inhibits the Dalton’s ascitic lymphoma in Swiss albino mice: A preliminary study exploring possible mechanism of action

    Bibin Baby Augustine1★, Suvakanta Dash2, Mangala Lahkar1, Usha Sarma3, Pavan Kumar Samudrala1, Jaya Mary Thomas4★ Corresponding author

    1. 1Laboratory of Tumor Pharmacology, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, India.
    2. 2Department of Pharmaceutics, Girijananda Institute of Pharmaceutical Sciences, Azara, Guwahati, India.
    3. 3Department of Pathology, Gauhati Medical College, Guwahati, Assam, India.
    4. 4Department of Pharmacology, College of Pharmaceutical Sciences, Medical College, Trivandrum, India.

    CORRESPONDENCE

    Bibin Baby Augustine

    Laboratory of Tumor Pharmacology, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, India.

    bibinbabyaugustine@gmail.com

    Received: 19-06-2013; Revised: 17-08-2013.

    Volume 10, Issue 38 · pp. 118–124 · PUBLISHED 17 April 2014 · DOI: 10.4103/0973-1296.131022

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: North East India is a rich source of medicinal plants and a number of plant extracts are used by tribal peoples living in this area for various disorders. L.aspera is such a plant, traditionally used as an antitumor agent. Aim: In the present study, aerial parts of L.aspera were investigated for antitumor activity in Dalton’s lymphoma (DAL) bearing mice. The ability of plant extract in free radical scavenging, neoangiogenesis inhibition and macrophage stimulation were also checked. Materials and Methods: Based on the preliminary in vitro cytotoxicity studies ethyl acetate fraction of L.aspera (EALA) was selected for the detailed study. DAL ascites tumor model was performed to check the antitumor activity of EALA (200 and 400mg/kg of body weight). Hematological and histopathological parameters were estimated. Antioxidant levels, neoangiogenesis and peritoneal macrophage count were also determined. Results: In vitro MTT and Trypan blue assay results showed the cytotoxic effect of EALA in DAL cells lines. EALA treatment resulted in signifi cant decrease in ascites tumor volume and viable cell count. Hematological and liver antioxidant parameters were normalised by EALA treatment. It was also found that EALA treatment inhibits neovascularisation and produce macrophage stimulation in treated mice. Conclusion: The results showed that EALA is a promising anticancer agent and its activity is comparable to the standard drug 5-Flouro uracil (5-FU).

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      Augustine, B. B., Dash, S., Lahkar, M., Sarma, U., Samudrala, P. K., & Thomas, J. M. (2014). Leucas aspera inhibits the Dalton’s ascitic lymphoma in Swiss albino mice: A preliminary study exploring possible mechanism of action. Pharmacognosy Magazine, 10(38), 118–124. https://doi.org/10.4103/0973-1296.131022