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    Article

    The extracts of Japanese willow tree species are effective forapoptotic desperation or differentiation of acute myeloid leukemia cells

    Kounosuke Fujita1, Yuji Nomura2, Masahiko Sawajiri3, Pravat K. Mohapatra4, Hany A. El-Shemy5★, Nguyen T. Nguyen6, Masashi Hosokawa6, Kazuo Miyashita6, Teruo Maeda6, Hirofumi Saneoka6, Shohei Fujita6, Takayuki Fujita6★ Corresponding author

    1. 1Fazenda Fuiita LLC, Nanaehama, Hokuto, 049-0111, Japan.
    2. 2Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima - 734-0037, Japan.
    3. 3School of Life Science, Sambalpur University, Jyoti Vihar, Sambalpur, India.
    4. 4Cairo Universty Research Park, Biochemistry Department, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.
    5. 5Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
    6. 6Graduate School of Fisheriers Sciences, Hokkaido University, Minato, Hakodate, 041-8611 Japan, Japan.

    CORRESPONDENCE

    Hany A. El-Shemy

    Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.

    helshemy@hotmail.com

    Received: 22-09-2012; Revised: 04-11-2012; Accepted: 04-11-2012.

    Volume 10, Issue 38 · pp. 125–126 · PUBLISHED 17 April 2014 · DOI: 10.4103/0973-1296.131023

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The antileukemic activity of hot water extract of plant parts of some Japanese willow tree species grown at different levels of nitrogen were examined. Materials and Methods: Water extracts of willow leaves were prepared for this studies in different level of nitrogen nutrition. Results: The extracts obtained from the leaves and stem exhibited anti-leukemic activities prominently. The crude hot water extracts of the young growing parts including apex, matured leaves and stem, killed the blasts of acute myeloid leukemia (AML) cells, (HL60 and NB4) after 48h incubation, however, such desperation was far less in the root extract. Similar to the plant parts, response of extracts obtained from different willow species was not identical; the proportion of dead cells relative to whole cells of the culture medium ranged from 21% to 93% among the species. Leaf extracts obtained from the responsive willow species decreased the live cell percentage and increased the dead cell percentage at higher level of nitrogen nutrition. The mode of desperation of leaf extract treated AML cells in such species appeared to be cell apoptosis as shown by binding with fl uorescein isothiocyanate (FITC) -labeled Annexin V. Conclusion: Differentiation of alive AML cells continued unabated and apoptosis was poor when extract of an unresponsive species added to the culture medium.

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      Fujita, K., Nomura, Y., Sawajiri, M., Mohapatra, P. K., El-Shemy, H. A., Nguyen, N. T., Hosokawa, M., Miyashita, K., Maeda, T., Saneoka, H., Fujita, S., & Fujita, T. (2014). The extracts of Japanese willow tree species are effective forapoptotic desperation or differentiation of acute myeloid leukemia cells. Pharmacognosy Magazine, 10(38), 125–126. https://doi.org/10.4103/0973-1296.131023