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    Inhibition of LDL oxidation and oxidized LDL‑induced foam cell formation in RAW 264.7 cells show anti‑atherogenic properties of a foliar methanol extract of Scoparia dulcis

    Sinjitha S. Nambiar1, Nandini Prasad Shetty1★, Praveena Bhatt2, Bhagyalakshmi Neelwarne1★ Corresponding author

    1. 1Department of Plant Cell Biotechnology, Institute (Council of Scientific and Industrial Research), Mysore, Karnataka, India.
    2. 2Fermentation Technology and Bioengineering, Central Food Technological Research Institute (Council of Scientific and Industrial Research), Mysore, Karnataka, India.

    CORRESPONDENCE

    Nandini Prasad Shetty

    Department of Plant Cell Biotechnology, Institute (Council of Scientific and Industrial Research), Mysore, Karnataka, India.

    pcbt@cftri.res.in

    Received: 04-07-2013; Revised: 02-08-2013.

    Volume 10, Issue 38s · pp. 240–248 · PUBLISHED 28 May 2014 · DOI: 10.4103/0973-1296.133241

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Oxidation of low density lipoproteins and their further uptake by macrophages is known to result in the formation of foam cells, which are critical in the initiation of atherosclerosis through activation of inflammatory signalling cascades. Thus, powerful dietary antioxidants are receiving attention for the reversal of such pathological states. Materials and Methods: Extracts of Scoparia dulcis have been used as tea and health drinks with various health promoting effects. In the present study, we examined the reactive oxygen scavenging potential as well as anti‑inflammatory and anti‑atherogenic efficacies, using leaf extracts obtained after successive extraction with various solvents. Results: A methanol extract showed potent antioxidant activity with an IC50 value of 570 μg/ml, caused hydrogen peroxide scavenging (28.9 μg/ml) and anti‑inflammatory effects by improving human erythrocyte membrane stabilisation (about 86%). The methanol extract also efficiently inhibited lipid peroxidation and oxidation of low density lipoproteins, thus preventing foam cell formation in cultured RAW 264.7 cells. Furthermore, phytochemical screening of the extracts showed high accumulation of flavonoids. Conclusions: The foliar methanol extract of Scoparia dulcis has a strong anti‑atherogenic potential and this property could be attributed maybe due to presence of flavonoids since HPLC analysis showed high concentrations of myricetin and rutin in the methanol extract.

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      Nambiar, S. S., Shetty, N. P., Bhatt, P., & Neelwarne, B. (2014). Inhibition of LDL oxidation and oxidized LDL‑induced foam cell formation in RAW 264.7 cells show anti‑atherogenic properties of a foliar methanol extract of Scoparia dulcis. Pharmacognosy Magazine, 10(38s), 240–248. https://doi.org/10.4103/0973-1296.133241