Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Mycelial fermentation characteristics and antiproliferative activity of Phellinus vaninii Ljup

    Wei Hu2,3, Shuai Liu4, Yuexin Zhang3, Deng Xun5, Chunping Xu4★★ Corresponding author

    1. 1Branch of Forest Protection, School of Forestry, Northeast Forestry University, China.
    2. 2Heilongjiang Forest By‑product and Specialty Institute, Mudanjiang 157011, China.
    3. 3College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002, China.
    4. 4Heilongjiang Forestry Protection Institute, Harbin 150040, China.

    CORRESPONDENCE

    Chunping Xu

    Heilongjiang Forestry Protection Institute, Harbin 150040, China.

    c.p.xu@zzuli.edu.cn

    Received: 17-12-2013; Revised: 27-03-2014.

    Volume 10, Issue 40 · pp. 430–434 · PUBLISHED 26 September 2014 · DOI: 10.4103/0973-1296.141812

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The mycelial fermentation of higher fungi were investigated to posses various bioactivities. Materials and Methods: The mycelial growth and pellet morphology in a 5-L bioreactor were investigated. The mycelial broth containing biomass and extracellular products harvested from the fermentor was tested for antiproliferative activity of colon cancer LoVo cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay. Results: The maximum mycelial concentration in a 5-L bioreactor was 12.5 g/L after 8 days cultivation. Further investigation in the mycelial pellets during the fermentation period revealed that the mean diameter of the pellet morphology was positively correlated with mycelial biomass (R2 = 0.82, P < 0.05) and broth viscosity (R2 = 0.90, P < 0.01), significantly. The ethyl acetate extract showed the most significant effects, increasing the inhibition rate up to 87.5% after 48 h at concentration of 1000 μg/mL. Conclusion: The results demonstrated the feasibility of P. vaninii Ljup mycelial fermentation for large-scale production of bioactive and medicinal compounds.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Hu, W., Liu, S., Zhang, Y., Xun, D., & Xu, C. (2014). Mycelial fermentation characteristics and antiproliferative activity of Phellinus vaninii Ljup. Pharmacognosy Magazine, 10(40), 430–434. https://doi.org/10.4103/0973-1296.141812