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    Article

    Er‑Xian Decoction, a traditional Chinese herbal formula, intervening early in hypothalamic‑pituitary axis of male rats with delayed puberty

    Zheng Zhu1, LiHong Li1, Xin Jin1, JianWei Fang1, DongFang Zhang1★★ Corresponding author

    1. 1Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR, China.

    CORRESPONDENCE

    DongFang Zhang

    Department of Pharmacognosy, School of Pharmacy, China Medical University, Shenyang, PR, China.

    lnsyzdf@sohu.com

    Received: 18-10-2013; Revised: 04-11-2013.

    Volume 10, Issue 40 · pp. 517–521 · PUBLISHED 26 September 2014 · DOI: 10.4103/0973-1296.141780

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Er‑Xian Decoction (EXD) is one of the traditional Chinese medicine (TCM) with unique effect on osteoporosis, menopausal syndrome and delayed puberty in China for many years. Objective: We aim to evaluate the potential activity of starting hypothalamic–pituitary–testicular (HPT) axis of male rats with delayed puberty. Materials and Methods: Delayed puberty model of male Sprague– Dawley (SD) rats were established with soy isoflavones (90 mg·kg‑1) and were treated by EXD extract at doses of 5, 10 g·kg‑1 or Testosterone undecanoate (TU) for 8 weeks. Body weight, body length, testis weight, T, E2 and luteinizing hormone (LH) in serum, gonadotropin releasing hormone (GnRH) in hypothalamus, follicle stimulating hormone (FSH) and LH in pituitary gland were determined by ELISA. Immunohistochemistry was used to detect LH in pituitary gland. Results: Soy isoflavones could significantly decrease body weight, body length, testicular organ coefficient T in serum, GnRH in hypothalamus, FSH and LH in pituitary gland. Both of EXD and TU could improve the condition. E2 and LH in serum of all groups were non‑significance of difference (P > 0.05). The immunohistochemical results were well consistent with LH in pituitary gland. Conclusion: The results of the present research indicate that EXD extract is effective to start the HPT axis in puberty and can significantly improve sexual developmental inhibition caused by soy isoflavones.

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      Zhu, Z., Li, L., Jin, X., Fang, J., & Zhang, D. (2014). Er‑Xian Decoction, a traditional Chinese herbal formula, intervening early in hypothalamic‑pituitary axis of male rats with delayed puberty. Pharmacognosy Magazine, 10(40), 517–521. https://doi.org/10.4103/0973-1296.141780