Quantitative analysis of the eight major compounds in the Samsoeum using a high‑performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometer
Jin Bae Weon2, Hye Jin Yang2, Bohyoung Lee2, Jin Yeul Ma3, Choong Je Ma2,4★★ Corresponding author
- 1Department of Biomaterials Engineering, Division of Bioscience and Biotechnology.
- 2TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong‑gu, Daejeon 305‑811, Korea.
- 3Research Institute of Biotechnology, Kangwon National University, Chuncheon 200‑701, Korea.
CORRESPONDENCE
Choong Je Ma
TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong‑gu, Daejeon 305‑811, Korea.
Received: 03-06-2014; Revised: 19-06-2014.
Volume 11, Issue 42 · pp. 320–326 · PUBLISHED 12 March 2015 · DOI: 10.4103/0973-1296.153085
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ABSTRACT
Background: Samsoeum was traditionally used for treatment of a respiratory disease. Objective: The simultaneous determination of eight major compounds, ginsenoside Rg3, caffeic acid, puerarin, costunolide, hesperidin, naringin, glycyrrhizin, and 6‑gingerol in the Samsoeum using a high‑performance liquid chromatography (HPLC) coupled with diode array detection (DAD) and an electrospray ionization mass spectrometer was developed for an accurate and reliable quality assessment. Materials and Methods: Eight compounds were qualitative identified based on their mass spectra and by comparing with standard compounds and quantitative analyzed by HPLC‑DAD. Separation of eight compounds was carried out on a LUNA C18 column (S‑5 µm, 4.6 mm i.d. ×250 mm) with gradient elution composed of acetonitrile and 0.1% trifluoroacetic acid. Results: The data showed good linearity (R2 > 0.9996). The limits of detection and the limits of quantification were <0.53 μg and 1.62 μg, respectively. Inter‑ and Intra‑day precisions (expressed as relative standard deviation values) were within 1.94% and 1.91%, respectively. The recovery of the method was in the range of 94.24–107.90%. Conclusion: The established method is effective and could be applied to quality control of Samsoeum.
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Weon, J. B., Yang, H. J., Lee, B., Ma, J. Y., & Ma, C. J. (2015). Quantitative analysis of the eight major compounds in the Samsoeum using a high‑performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometer. Pharmacognosy Magazine, 11(42), 320–326. https://doi.org/10.4103/0973-1296.153085