Profiling of components of rhizoma et radix polygoni cuspidati by high-performance liquid chromatography with ultraviolet diode-array detector and ion trap/time-of-flight mass spectrometric detection
Jinfeng Fu1, Min Wang1, Huimin Guo1, Yuan Tian1, Zunjian Zhang1★, Rui Song1,2★★ Corresponding author
- 1Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, China.
- 2Department of Pharmaceutical Analysis, China Pharmaceutical University, Tongjia Lane 24, Nanjing 210009, China.
CORRESPONDENCE
Zunjian Zhang
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, China.
Received: 27-07-2014; Revised: 18-09-2014; Accepted: 18-09-2014.
Volume 11, Issue 43 · pp. 486–493 · PUBLISHED 10 July 2015 · DOI: 10.4103/0973-1296.160455
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ABSTRACT
Introduction: Rhizoma et Radix Polygoni Cuspidati (Huzhang in Chinese, HZ) is a traditional medicinal plant in China. Many of the components of HZ have been proved to be bioactive while it is difficult to conduct a comprehensive chemical profiling of HZ as a consequence of the absence of efficient separation system and sensitive detective means. We developed a simple and effective method for comprehensive characterization of constituents in HZ. Objective: To develop a simple and effective method to characterize the components in HZ and provide useful information for subsequent metabolic studies of HZ. Materials and Methods: The components in HZ aqueous extract were characterized by using high performance liquid chromatography with UV diode-array detector (HPLC-DAD) and ion trap/time-of-flight mass spectrometric detection (HPLC-IT/TOF). Stilbenes, anthraquinones, gallates and tannins, naphthalenes and some other compounds were identified and confirmed by diagnostic fragment ions with accurate mass measurements, characteristic fragmentation pathways and relevant published literatures. Results: Among the 238 constituents detected in HZ, a total number of 74 constituents were identified unambiguously or tentatively, including 29 compounds reported for the first time in HZ. Conclusion: The identification and structure elucidation of these chemicals provided essential data for quality control and further in vivo metabolic studies of HZ.
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Fu, J., Wang, M., Guo, H., Tian, Y., Zhang, Z., & Song, R. (2015). Profiling of components of rhizoma et radix polygoni cuspidati by high-performance liquid chromatography with ultraviolet diode-array detector and ion trap/time-of-flight mass spectrometric detection. Pharmacognosy Magazine, 11(43), 486–493. https://doi.org/10.4103/0973-1296.160455