Quantification of maltol in Korean ginseng (Panax ginseng) products by high‑performance liquid chromatography‑diode array detector
Hyun Cheol Jeong1, Hee‑Do Hong1, Young‑Chan Kim1, Young Kyoung Rhee1, Sang Yoon Choi1, Kyung‑Tack Kim1, Sung Soo Kim1, Young‑Chul Lee1, Chang‑Won Cho1★★ Corresponding author
- 1Ginseng Research Center, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea.
CORRESPONDENCE
Chang‑Won Cho
Ginseng Research Center, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea.
Received: 16-07-2014; Revised: 13-08-2014.
Volume 11, Issue 43 · pp. 657–664 · PUBLISHED 10 July 2015 · DOI: 10.4103/0973-1296.160452
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ABSTRACT
Background: Maltol, as a type of phenolic compounds, is produced by the browning reaction during the high‑temperature treatment of ginseng. Thus, maltol can be used as a marker for the quality control of various ginseng products manufactured by high‑temperature treatment including red ginseng. For the quantification of maltol in Korean ginseng products, an effective high‑performance liquid chromatography‑diode array detector (HPLC‑DAD) method was developed. Materials and Methods: The HPLC‑DAD method for maltol quantification coupled with a liquid‑liquid extraction (LLE) method was developed and validated in terms of linearity, precision, and accuracy. An HPLC separation was performed on a C18 column. Results: The LLE methods and HPLC running conditions for maltol quantification were optimized. The calibration curve of the maltol exhibited good linearity (R2 = 1.00). The limit of detection value of maltol was 0.26 µg/mL, and the limit of quantification value was 0.79 µg/mL. The relative standard deviations (RSDs) of the data of the intra‑ and inter‑day experiments were <1.27% and 0.61%, respectively. The results of the recovery test were 101.35–101.75% with an RSD value of 0.21–1.65%. The developed method was applied successfully to quantify the maltol in three ginseng products manufactured by different methods. Conclusion: The results of validation demonstrated that the proposed HPLC‑DAD method was useful for the quantification of maltol in various ginseng products.
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Jeong, H. C., Hong, H., Kim, Y., Rhee, Y. K., Choi, S. Y., Kim, K., Kim, S. S., Lee, Y., & Cho, C. (2015). Quantification of maltol in Korean ginseng (Panax ginseng) products by high‑performance liquid chromatography‑diode array detector. Pharmacognosy Magazine, 11(43), 657–664. https://doi.org/10.4103/0973-1296.160452