Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Poncirus trifoliata Rafin. induces the apoptosis of triple‑negative breast cancer cells via activation of the c‑Jun NH(2)‑terminal kinase and extracellular signal‑regulated kinase pathways

    Hye‑Yeon Han1★, Mi Heon Ryu1★, Yonghae Son2, Guemsan Lee3, Seung-Hwa Jeong4, Hyungwoo Kim2★★ Corresponding author

    1. 1Department of Oral Pathology, School of Dentistry, Institute of translational Dental Sciences, South Korea.
    2. 2Division of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, South Korea.
    3. 3Department of Herbology, College of Korean Medicine, Wonkwang, University, Iksan, South Korea.
    4. 4Department of Preventive and Community Dentistry, School of Dentistry, Pusan National University, Yangsan, South Korea.

    CORRESPONDENCE

    Hye‑Yeon Han

    Department of Oral Pathology, School of Dentistry, Institute of translational Dental Sciences, South Korea.

    kronos7@pusan.ac.kr

    Received: 18-09-2014; Revised: 29-10-2014.

    Volume 11, Issue 44s1 · pp. 237–243 · PUBLISHED 24 September 2015 · DOI: 10.4103/0973-1296.166056

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Poncirus trifoliata Rafin. is a traditional medicine with known anti‑inflammatory and anti‑cancer properties. Traditionally, it is used to control chronic inflammation, allergy and gastrointestinal diseases such as digestive ulcers gastritis in China, Japan, and Korea. Objectives: To evaluate the apoptosis‑inducing activity of a P. trifoliata methanol extract (MEPT) and elucidate the molecular mechanisms. Materials and Methods: The anti‑cancer effect of MEPT and its underlying mechanisms were investigated in breast cancer cells using 3,4,5‑dimethyl N‑methylthiazol‑2‑yl‑2, 5‑d‑phenyl tetrazolium bromide assay, cell cycle analysis, and western blotting. Results: MEPT suppressed the proliferation of MDA‑MB‑231 cells with inhibition dose 50% value of 119.44 μg/mL at 24 h, which have features typical of triple‑negative breast cancer cells. MEPT also altered the characteristic features of the MDA‑MB‑231 cells and increased the proportion of cells undergoing sub‑G1 arrest. In addition, MEPT increased levels of caspase 8 and 3 in MDA‑MB‑231 cells, whereas caspase 9 was not detected. In addition, MEPT‑induced tumor necrosis factor receptor (TNFR) and TNFR type 1‑associated death domain (TRADD) protein and the activations of c‑Jun NH(2)‑terminal kinase (JNK) and extracellular signal‑regulated kinases (ERK). Conclusion: Our results indicate that MEPT has chemotherapeutic potential in triple‑negative breast cancer and that at the molecular level its effects are derived from the activations of TNFR and of the mitogen‑activated protein kinase pathway.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Han, H., Ryu, M. H., Son, Y., Lee, G., Jeong, S., & Kim, H. (2015). Poncirus trifoliata Rafin. induces the apoptosis of triple‑negative breast cancer cells via activation of the c‑Jun NH(2)‑terminal kinase and extracellular signal‑regulated kinase pathways. Pharmacognosy Magazine, 11(44s1), 237–243. https://doi.org/10.4103/0973-1296.166056