Reversed‑phase high‑performance Liquid Chromatography‑ ultraviolet Photodiode Array Detector Validated Simultaneous Quantification of six Bioactive Phenolic Acids in Roscoea purpurea Tubers and their In vitro Cytotoxic Potential against Various Cell Lines
Sharad Srivastava1★, Ankita Misra1, Dharmesh Kumar2, Amit Srivastava1, Anil Sood2, AKS Rawat1★ Corresponding author
- 1Pharmacognosy and Ethnopharmacology Division, CSIR‑National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
- 2Biotechnology Division, CSIR‑Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India.
CORRESPONDENCE
Sharad Srivastava
Pharmacognosy and Ethnopharmacology Division, CSIR‑National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Volume 11, Issue 44s2 · pp. 488–495 · PUBLISHED 2015 · DOI: 10.4103/0973-1296.168944
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Roscoea purpurea or Roscoea procera Wall. (Zingiberaceae) is traditionally used for nutrition and in the treatment of various ailments. Objective: Simultaneous reversed‑phase high‑performance liquid chromatography‑ultraviolet (RP‑HPLC) photodiode array detector identification of phenolic acids (PA’s) was carried out in whole extract of tuber and their cytotoxic potential was estimated along with radical scavenging action. Bioactivity guided fractionation was also done to check the response potential against the same assay. Materials and Methods: Identification and method validation was performed on RP‑HPLC column and in vitro assays were used for bioactivity. Results: Protocatechuic acid, syringic acid, ferulic acid, rutin, apigenin, and kaempferol were quantified as 0.774%, 0.064%, 0.265%, 1.125%, 0.128%, and 0.528%, respectively. Validated method for simultaneous determination of PA’s was found to be accurate, reproducible, and linearity was observed between peak area response and concentration. Recovery of identified PA’s was within the acceptable limit of 97.40–104.05%. Significant pharmacological response was observed in whole extract against in vitro cytotoxic assay, that is, Sulforhodamine B assay, however, fractionation results in decreased action potential. Similar pattern of results were observed in the antioxidant assay, as total phenolic content and total flavonoid content were highest in whole extract and decreases with fractionation. Radical scavenging activity was prominent in chloroform fraction, exhibiting IC50 at 0.25 mg/ mL. Conclusion: Study, thus, reveals that R. purpurea exhibit significant efficacy in cytotoxic activity with the potentiality of scavenging free radicals due the presence of PA’s as reported through RP‑HPLC.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Srivastava, S., Misra, A., Kumar, D., Srivastava, A., Sood, A., & Rawat, A. (2015). Reversed‑phase high‑performance Liquid Chromatography‑ ultraviolet Photodiode Array Detector Validated Simultaneous Quantification of six Bioactive Phenolic Acids in Roscoea purpurea Tubers and their In vitro Cytotoxic Potential against Various Cell Lines. Pharmacognosy Magazine, 11(44s2), 488–495. https://doi.org/10.4103/0973-1296.168944