Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Cucumis melo ssp. Agrestis var. Agrestis Ameliorates High Fat Diet Induced Dyslipidemia in Syrian Golden Hamsters and Inhibits Adipogenesis in 3T3‑L1 Adipocytes

    Kripa Shankar2, Sumit K. Singh3, Durgesh Kumar2,4, Salil Varshney2, Abhishek Gupta2, Sujith Rajan2,4, Ankita Srivastava2,4, Muheeb Beg2, Anurag Kumar Srivastava5, Sanjeev Kanojiya6, Dipak K. Mishra2,3, Anil N. Gaikwad2★★ Corresponding author

    1. 1Division of Pharmacology, CSIR‑Central Drug Research Institute, India.
    2. 2Division of Botany, CSIR‑Central Drug Research Institute, India.
    3. 3Academy of Scientific and Innovative Research, CSIR‑Central Drug Research Institute, India.
    4. 4Division of Toxicology, CSIR‑Central Drug Research Institute, India.
    5. 5Sophisticated Analytical Instrument Facility, CSIR‑Central Drug Research Institute, Lucknow, Uttar Pradesh, India.

    CORRESPONDENCE

    Anil N. Gaikwad

    Division of Botany, CSIR‑Central Drug Research Institute, India.

    anil_gaikwad@cdri.res.in

    Volume 11, Issue 44s3 · pp. 501–510 · PUBLISHED 2015 · DOI: 10.4103/0973-1296.172945

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Cucumis melo ssp. agrestis var. agrestis (CMA) is a wild variety of C. melo. This study aimed to explore anti‑dyslipidemic and anti‑adipogenic potential of CMA. Materials and Methods: For initial anti‑dyslipidemic and antihyperglycemic potential of CMA fruit extract (CMFE), male Syrian golden hamsters were fed a chow or high‑fat diet with or without CMFE (100 mg/kg). Further, we did fractionation of this CMFE into two fractions namely; CMA water fraction (CMWF) and CMA hexane fraction (CMHF). Phytochemical screening was done with liquid chromatography‑mass spectrometry LC- (MS)/MS and direct analysis in real time‑MS to detect active compounds in the fractions. Further, high‑fat diet fed dyslipidemic hamsters were treated with CMWF and CMHF at 50 mg/kg for 7 days. Results: Oral administration of CMFE and both fractions (CMWF and CMHF) reduced the total cholesterol, triglycerides, low‐density lipoprotein cholesterol, and very low‐density lipoprotein‑cholesterol levels in high fat diet‑fed dyslipidemic hamsters. CMHF also modulated expression of genes involved in lipogenesis, lipid metabolism, and reverse cholesterol transport. Standard biochemical diagnostic tests suggested that neither of fractions causes any toxicity to hamster liver or kidneys. CMFE and CMHF also decreased oil‑red‑O accumulation in 3T3‑L1 adipocytes. Conclusion: Based on these results, it is concluded that CMA possesses anti‑dyslipidemic and anti‑hyperglycemic activity along with the anti‑adipogenic activity.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Shankar, K., Singh, S. K., Kumar, D., Varshney, S., Gupta, A., Rajan, S., Srivastava, A., Beg, M., Srivastava, A. K., Kanojiya, S., Mishra, D. K., & Gaikwad, A. N. (2015). Cucumis melo ssp. Agrestis var. Agrestis Ameliorates High Fat Diet Induced Dyslipidemia in Syrian Golden Hamsters and Inhibits Adipogenesis in 3T3‑L1 Adipocytes. Pharmacognosy Magazine, 11(44s3), 501–510. https://doi.org/10.4103/0973-1296.172945