H2O2 Improves Quality of Radix scutellariae Through Anti-oxidant Effect
Song Qi1, Cao Wu-lin1, Jiang Hua1,2, Zhang Ai-hua1, Meng Xiang-cai1★★ Corresponding author
- 1Department of Pharmacognosy, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
- 2Department of Pharmacognosy, College of Pharmacy, Liaoning Medical University, Jinzhou 121000, China.
CORRESPONDENCE
Meng Xiang-cai
Department of Pharmacognosy, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Volume 12, Issue 45 · pp. 84–90 · PUBLISHED 2016 · DOI: 10.4103/0973-1296.176063
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ABSTRACT
Introduction: The correlation between the quality and geographical origin of herbal medicine was traced back to Tang Dynasty in China, more than 1200 years, and the effects of ecological environments on the secondary metabolites such as flavonoids have been confirmed. However, little is known about how the adversity impacts on the quality. Reactive oxygen species (ROS) may be medium between the ecological environment and the secondary metabolism. Materials and Methods: The fresh roots of Scutellaria baicalensis Georgi were treated with 0.002 μmol/L, 0.2 μmol/L, and 20 μmol/L H2O2, respectively. A stress model was established to elucidate the change of secondary metabolism, anti-oxidant enzyme system, and enzymes relating to flavonoids. Results: The activities of superoxide dismutase, catalase and peroxidase decreased. Too much H2O2, firstly, boosted transformation of flavonoids glycoside into aglucon with the most remarkable activities through UDP-glucuronate baicalein 7-O-glucuronosyltransferase (UBGAT), and β-glucuronidase (GUS), then regulated the gene expression of phenylalanine ammonialyase, GUS, and UBGAT, and increased the contents of flavones, motivated the flavonoid glycoside converting into aglucon. With this action, the flavones displaced the anti-oxidant enzymes. The higher the dosage, the more baicalein and wogonin increased, the later they took action. Conclusion: The plant secondary metabolites to keep ROS constant are identical to the effective materials in clinic. They are closely linked. H2O2 can improve flavones, especially the aglucon, and further increased the quality of herbal medicine, which possesses very important value in medical practice.
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Qi, S., Wu-lin, C., Hua, J., Ai-hua, Z., & Xiang-cai, M. (2016). H2O2 Improves Quality of Radix scutellariae Through Anti-oxidant Effect. Pharmacognosy Magazine, 12(45), 84–90. https://doi.org/10.4103/0973-1296.176063