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    Article

    In vitro and In vivo Antioxidant Activity of Flavonoid Extracted from Mulberry Fruit (Morus alba L.)

    Sivakumar Thasma Raman1, Ajay Krishna Palani Gounder Ganeshan1, Cheng Chen1, Chao Jin1, Shao-Hui Li1, Hui-Juan Chen1, Zhongzheng Gui1★★ Corresponding author

    1. 1School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, P.R. China.

    CORRESPONDENCE

    Zhongzheng Gui

    School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, P.R. China.

    srizzgui@hotmail.com

    Received: 14-10-2015; Revised: 09-11-2015; Accepted: 09-11-2015.

    Volume 12, Issue 46 · pp. 128–133 · PUBLISHED 2 March 2016 · DOI: 10.4103/0973-1296.177910

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Many plants possess antioxidants that exhibit additive or synergistic activities. Objective: In this study, an ethanol-extracted flavonoid extracted from mulberry fruit (FEM) was evaluated for the antioxidant activity in vitro and the hemolysis in red blood cell (RBC) and lipid peroxidation in liver in vivo. Materials and Methods: Antioxidant activities in vitro were measured by quantifying its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, reducing power, and Fe2+-chelating ability. FEM inhibits hemolysis in RBCs and effects of lipid peroxidation in the liver were estimated. Results: The total content of flavonoid compounds was 187.23 mg of quercetin equivalents per grams dried material. In the in vitro assays, FEM demonstrated a strong antioxidant effect, especially in DPPH scavenging activity and reducing power. Mouse RBC hemolysis induced by H2O2 was significantly inhibited by FEM in a dose- and time-dependent manner. The effects of FEM on lipid peroxidation in liver, mitochondria, and microsome were investigated. The percentage of inhibition at high concentration (100 µg/mL) of FEM was 45.51%, 39.36%, and 42.78% for liver, mitochondria, and microsomes, respectively. These results suggest that the FEM possesses a strong antioxidant activity both in vivo and in vitro.

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      Raman, S. T., Ganeshan, A. K. P. G., Chen, C., Jin, C., Li, S., Chen, H., & Gui, Z. (2016). In vitro and In vivo Antioxidant Activity of Flavonoid Extracted from Mulberry Fruit (Morus alba L.). Pharmacognosy Magazine, 12(46), 128–133. https://doi.org/10.4103/0973-1296.177910